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Product Info Summary
| SKU: | A01268-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-PDK1 Antibody
View all Pyruvate Dehydrogenase Kinase 1/PDK1 Antibodies
SKU/Catalog Number
A01268-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PDK1 Antibody catalog # A01268-2. Tested in WB,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01268-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ACBP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01268-2 is reactive to PDK1 in Human, Mouse, Rat
Applications
A01268-2 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
49.244kDa
Background of Pyruvate Dehydrogenase Kinase 1/PDK1
Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress By similarity.
Delia J. Hern¨¢ndez-Deviez, Hum. Mol. Genet., Jan 2006; 15: 129 - 142.
Yanchao Huang, FASEB J, Mar 2007; 21: 732 - 742.
Mengfatt Ho, Hum. Mol. Genet., Sep 2004; 13: 1999 - 2010.
Eriko Fujita, Hum. Mol. Genet., Mar 2007; 16: 618 - 629.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of PDK1 using Anti-PDK1 Antibody (A01268-2).
ICC staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-PDK1 Antibody A01268-2
Western blot analysis of PDK1 on rat heart tissue lysate using anti-PDK1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For PDK1 (Source: Uniprot.org, NCBI)
Gene Name
PDK1
Full Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
Weight
49.244kDa
Superfamily
PDK/BCKDK protein kinase family
Alternative Names
EC 2.7.11; EC 2.7.11.2; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; PDK1; PDK-1; PKB kinase; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1; Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial PDK1 pyruvate dehydrogenase kinase 1 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial|pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial|PDH kinase 1|mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDK1, check out the PDK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDK1 Antibody (A01268-2)
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No publications found for A01268-2
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