Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband™

Product Name

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband™

View all Pyruvate Dehydrogenase Kinase 1/PDK1 Antibodies

SKU/Catalog Number

A01268-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband™ catalog # A01268-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01268-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human PDK1 recombinant protein (Position: V107-Y336).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01268-1 is reactive to PDK1 in Human, Rat

Applications

A01268-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

49.244kDa

Background of Pyruvate Dehydrogenase Kinase 1/PDK1

PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PDK1 using anti-PDK1 antibody (A01268-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK1 antigen affinity purified polyclonal antibody (Catalog # A01268-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK1 at approximately 58 kDa. The expected band size for PDK1 is at 49 kDa.

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Figure 2. IHC analysis of PDK1 using anti-PDK1 antibody (A01268-1).
PDK1 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDK1 Antibody (A01268-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For PDK1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.2; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; PDK1; PDK-1; PKB kinase; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1; Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial PDK1 pyruvate dehydrogenase kinase 1 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial|pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial|PDH kinase 1|mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1

For more info on PDK1, check out the PDK1 Infographic

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In this infographic, you will see the following information for PDK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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