This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CD274 antibody (M00109-2) performs well in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Flow cytometry analysis of PD-L1 overexpressing HEK293 cells using PD-L1 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L1 overexpressing HEK293 cells.
Bundle Save
Anti-PDL1 CD274 Monoclonal Antibody [4F2]
CD274 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | ELISA, Flow Cytometry, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-PDL1 CD274 Monoclonal Antibody [4F2]
View all CD274 Antibodies
View all CD274 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Mouse
Contents
PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 4F2
Isotype
IgG1
Immunogen
PD-L1 antibody was raised against the extracellular domain of human PD-L1.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
33.3 kDa
Background of CD274
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2). PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3). This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4).
Cite This Product
Anti-PDL1 CD274 Monoclonal Antibody [4F2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00109-2)
Review
Have you used Anti-PDL1 CD274 Monoclonal Antibody [4F2]?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-PDL1 CD274 Monoclonal Antibody [4F2] performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00109-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 17, 2026