Click image to see more details
Product Info Summary
| SKU: | A04832-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PDLIM1/Clim1 Antibody
SKU/Catalog Number
A04832-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PDLIM1/Clim1 Antibody (Catalog # A04832-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDLIM1/Clim1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04832-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PDLIM1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04832-1 is reactive to PDLIM1 in Human, Mouse, Rat
Applications
A04832-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
36.072kDa
Background of PDLIM1
Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Kotaka M., J. Cell. Biochem. 72:279-285(1999).
Bauer K., Blood 96:4236-4245(2000).
Deloukas P., Nature 429:375-381(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human heart tissue using PDLIM1 antibody A04832-1.
Click image to see more details
Western blot analysis of extracts from Jurkat cells, COLO205 cells, 293 cells, HepG2 cells and HUVEC cells, using PDLIM1 antibody A04832-1.
Click image to see more details
Immunofluorescence analysis of HepG2 cells, using PDLIM1 antibody A04832-1.
Protein Target Info & Infographic
Gene/Protein Information For PDLIM1 (Source: Uniprot.org, NCBI)
Gene Name
PDLIM1
Full Name
PDZ and LIM domain protein 1
Weight
36.072kDa
Alternative Names
CLIM1carboxyl terminal LIM domain protein 1; CLP-36; CLP36carboxy terminal LIM domain protein 1; C-terminal LIM domain protein 1; elfin; enigma; hCLIM1; LIM domain protein CLP-36; PDZ and LIM domain 1 (elfin); PDZ and LIM domain 1; PDZ and LIM domain protein 1 PDLIM1 CLIM1, CLP-36, CLP36, HEL-S-112, hCLIM1 PDZ and LIM domain 1 PDZ and LIM domain protein 1|LIM domain protein CLP-36|carboxyl terminal LIM domain protein 1|elfin|epididymis secretory protein Li 112
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDLIM1, check out the PDLIM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDLIM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDLIM1/Clim1 Antibody (A04832-1)
Hello CJ!
No publications found for A04832-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PDLIM1/Clim1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-PDLIM1/Clim1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-PDLIM1/Clim1 Antibody
Question
We are currently using anti-PDLIM1/Clim1 antibody A04832-1 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-PDLIM1/Clim1 antibody (A04832-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
Is a blocking peptide available for product anti-PDLIM1/Clim1 antibody (A04832-1)?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We do provide the blocking peptide for product anti-PDLIM1/Clim1 antibody (A04832-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-31
Question
I was wanting to use your anti-PDLIM1/Clim1 antibody for IF for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
As indicated on the product datasheet, A04832-1 anti-PDLIM1/Clim1 antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
Do you have a BSA free version of anti-PDLIM1/Clim1 antibody A04832-1 available?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PDLIM1/Clim1 antibody A04832-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-27
Question
Would anti-PDLIM1/Clim1 antibody A04832-1 work for IF with cervix carcinoma erythroleukemia?
M. Singh
Verified customer
Asked: 2019-08-20
Answer
According to the expression profile of cervix carcinoma erythroleukemia, PDLIM1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-PDLIM1/Clim1 antibody A04832-1 will work for IF with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-08-20
Question
I see that the anti-PDLIM1/Clim1 antibody A04832-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-28
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-PDLIM1/Clim1 antibody A04832-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-05
Question
Our lab want to know about to test anti-PDLIM1/Clim1 antibody A04832-1 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-PDLIM1/Clim1 antibody A04832-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
The products we sell, including anti-PDLIM1/Clim1 antibody A04832-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-25
Question
Is this A04832-1 anti-PDLIM1/Clim1 antibody reactive to the isotypes of PDLIM1?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
The immunogen of A04832-1 anti-PDLIM1/Clim1 antibody is Synthesized peptide derived from internal of human PDLIM1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-18
Question
Can you help my question with product A04832-1, anti-PDLIM1/Clim1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04832-1 anti-PDLIM1/Clim1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-02
Question
Will anti-PDLIM1/Clim1 antibody A04832-1 work on primate WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
Our lab technicians have not tested anti-PDLIM1/Clim1 antibody A04832-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PDLIM1/Clim1 antibody A04832-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-PDLIM1/Clim1 antibody A04832-1. Let me know if you need anything else.
A. Krishna
Verified customer
Asked: 2017-05-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-03
Question
Would A04832-1 anti-PDLIM1/Clim1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Thomas
Verified customer
Asked: 2016-06-20
Answer
It shows on the product datasheet, A04832-1 anti-PDLIM1/Clim1 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-06-20
