Anti-PDS5B Antibody Picoband™

Product Name

Anti-PDS5B Antibody Picoband™

View all APRIN Antibodies

SKU/Catalog Number

A04823-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PDS5B Antibody Picoband™ catalog # A04823-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PDS5B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04823-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PDS5B recombinant protein (Position: Q499-Q1424).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04823-1 is reactive to PDS5B in Human, Mouse

Applications

A04823-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

170 kDa

Calculated molecular weight

164.667kDa

Background of APRIN

Sister chromatid cohesion protein PDS5 homolog B (PDS5B) is a protein that in humans is encoded by the PDS5B gene. This gene encodes a protein that interacts with the conserved protein complex termed cohesin. The cohesin complex holds together sister chromatids and facilitates accurate chromosome segregation during mitosis and meiosis. This protein is also a negative regulator of cell proliferation and may be a tumor-suppressor gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse
Flow Cytometry, 1-3 µg/1x1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PDS5B using anti-PDS5B antibody (A04823-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDS5B antigen affinity purified polyclonal antibody (Catalog # A04823-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDS5B at approximately 170 kDa. The expected band size for PDS5B is at 165 kDa.

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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-PDS5B antibody (A04823-1).
Overlay histogram showing PC-3 cells stained with A04823-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDS5B Antibody (A04823-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PDS5B (Source: Uniprot.org, NCBI)

Alternative Names

Androgen-induced proliferation inhibitorKIAA0979androgen induced inhibitor of proliferation; Androgen-induced prostate proliferative shutoff-associated protein AS3; androgen-induced shutoff 3; AS3APRIN; CG008; FLJ23236; PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae); sister chromatid cohesion protein PDS5 homolog B PDS5B APRIN, AS3, CG008 PDS5 cohesin associated factor B sister chromatid cohesion protein PDS5 homolog B|androgen induced inhibitor of proliferation|androgen-induced proliferation inhibitor|androgen-induced prostate proliferative shutoff-associated protein AS3|androgen-induced shutoff 3

For more info on PDS5B, check out the PDS5B Infographic

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In this infographic, you will see the following information for PDS5B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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