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Product Info Summary
| SKU: | M02530 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PELP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02530
BM4162 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PELP1 Rabbit Monoclonal Antibody catalog # M02530. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PELP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02530)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
COD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PELP1
Reactive Species
M02530 is reactive to PELP1 in Human
Observed Molecular Weight
160 kDa
Calculated molecular weight
119.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02530 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
IHC: mouse kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of PELP1 expression in 293T cell lysate.
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Immunohistochemical analysis of paraffin-embedded mouse kidney, using PELP1 Antibody.
Specific Publications For Anti-PELP1 Rabbit Monoclonal Antibody (M02530)
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Customer Q&As
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5 Customer Q&As for Anti-PELP1 Rabbit Monoclonal Antibody
Question
Does Abways provide a blocking peptide for M02530 | CY5284 ? If so, what is the cost?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Abways doesn't provide a
Boster Scientific Support
Answered: 2019-11-13
Question
My question regarding product M02530, anti-PELP1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02530 anti-PELP1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-08
Question
I was wanting to use your anti-PELP1 Rabbit Monoclonal antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
It shows on the product datasheet, M02530 anti-PELP1 Rabbit Monoclonal antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
We are currently using anti-PELP1 Rabbit Monoclonal antibody M02530 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
C. Krishna
Verified customer
Asked: 2017-10-24
Answer
The anti-PELP1 Rabbit Monoclonal antibody (M02530) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-24
Question
I am wondering do you have the information about the epitope of the anti-PELP1 rabbit monoclonal antibody M02530?
Verified Customer
Verified customer
Asked: 2013-11-15
Answer
The immunogen sequence for M02530 is AAGPPPQELVEEEP.
Boster Scientific Support
Answered: 2013-11-15
