Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband™

Product Name

Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband™

View all Peroxiredoxin 4 Antibodies

SKU/Catalog Number

PB9383

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband™ catalog # PB9383. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9383)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 4, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9383 is reactive to PRDX4 in Human, Mouse, Rat

Applications

PB9383 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

31 kDa

Calculated molecular weight

30.54kDa

Background of Peroxiredoxin 4

PRDX4 (peroxiredoxin 4) is also known as AOE37-2. The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. Functional analysis showed that PRDX4 protects glutamine synthetase from inactivation. Yeast 2-hybrid, immunoprecipitation, and immunoblot analyses indicated that PRDX4 and PRDX1 are capable of homodimerization and heterodimerization with each other but not with the mitochondrial PRDX3. Gel mobility shift and immunoblot analysis found that PRDX4 depletes NFKB binding activity together with a reduction in the amounts of p50, p65, and phosphorylated IKBA, as well as a reduction in the expression of HIV-1 viral proteins. Expression of PRDX4, alone or with PRDX1, increased the resistance of yeast cells to oxidant-induced toxicity. Jin et al. suggested PRDX4 modulates IKBA phosphorylation in the cytoplasm and thus affects a peroxiredoxin-dependent redox step.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Anti-Peroxiredoxin 4 Picoband antibody, PB9383, Western blotting
All lanes: Anti Peroxiredoxin 4 (PB9383) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Observed bind size: 31KD

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Anti-Peroxiredoxin 4 Picoband antibody, PB9383, IHC(P)
IHC(P): Human Tonsil Tissue

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Anti-Peroxiredoxin 4 Picoband antibody, PB9383, IHC(P)
IHC(P): Rat Brain Tissue

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Anti-Peroxiredoxin 4 Picoband antibody, PB9383, IHC(P)
IHC(P): Mouse Brain Tissue

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Figure 5. IHC analysis of Peroxiredoxin 4 using anti-Peroxiredoxin 4 antibody (PB9383).
Peroxiredoxin 4 was detected in immunocytochemical section of A549 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Peroxiredoxin 4 Antibody (PB9383) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 6. IF analysis of Peroxiredoxin 4 using anti-Peroxiredoxin 4 antibody (PB9383).
Peroxiredoxin 4 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Peroxiredoxin 4 Antibody (PB9383) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-Peroxiredoxin 4 antibody (PB9383).
Overlay histogram showing MCF-7 cells stained with PB9383 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 4 Antibody (PB9383,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PRDX4 (Source: Uniprot.org, NCBI)

Alternative Names

Antioxidant enzyme AOE372; AOE372; AOE37-2PRX-4; EC 1.11.1.15; Peroxiredoxin 4; Peroxiredoxin IV; peroxiredoxin-4; PRDX4; Prx4; prx-4; prx-IV; thioredoxin peroxidase (antioxidant enzyme); Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372 PRDX4 AOE37-2, AOE372, HEL-S-97n, PRX-4 peroxiredoxin 4 peroxiredoxin-4|antioxidant enzyme AOE372|epididymis secretory sperm binding protein Li 97n|peroxiredoxin IV|prx-IV|thioredoxin peroxidase (antioxidant enzyme)|thioredoxin peroxidase AO372|thioredoxin-dependent peroxide reductase A0372|thioredoxin-dependent peroxiredoxin 4

For more info on PRDX4, check out the PRDX4 Infographic

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In this infographic, you will see the following information for PRDX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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