Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband™

Product Name

Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband™

View all Peroxiredoxin 5 Antibodies

SKU/Catalog Number

PB9384

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband™ catalog # PB9384. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9384)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198). Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9384 is reactive to PRDX5 in Human, Mouse, Rat

Applications

PB9384 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

22.086kDa

Background of Peroxiredoxin 5

PRDX5 (peroxiredoxin 5) also known as AOEB166, ACR1,B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. The PRDX5 gene is mapped to11q13.1. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. PRDX5 plays a protective role against oxidative stress in human cartilage.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Anti-Peroxiredoxin 5 Picoband antibody, PB9384, IHC(P)
IHC(P): Human Lung Cancer Tissue

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Anti-Peroxiredoxin 5 Picoband antibody, PB9384, IHC(P)
IHC(P): Rat Intestine Tissue

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Anti-Peroxiredoxin 5 Picoband antibody, PB9384, IHC(P)
IHC(P): Mouse Intestine Tissue

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Anti-Peroxiredoxin 5 Picoband antibody, PB9384, Western blotting
All lanes: Anti Peroxiredoxin 5 (PB9384) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD

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Figure 5. IHC analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody (PB9384).
Peroxiredoxin 5 was detected in immunocytochemical section of SMMC Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Peroxiredoxin 5 Antibody (PB9384) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 6. IF analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody (PB9384).
Peroxiredoxin 5 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Peroxiredoxin 5 Antibody (PB9384) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 7. Flow Cytometry analysis of A549 cells using anti-Peroxiredoxin 5 antibody (PB9384).
Overlay histogram showing A549 cells stained with PB9384 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 5 Antibody (PB9384,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PRDX5 (Source: Uniprot.org, NCBI)

Alternative Names

ACR1; ACR1Peroxisomal antioxidant enzyme; Alu corepressor 1; Alu co-repressor 1; Antioxidant enzyme B166; AOEB166; AOEB166Peroxiredoxin V; AOPP; B166; EC 1.11.1.15; Liver tissue 2D-page spot 71B; MGC117264; MGC142283; MGC142285; Peroxiredoxin 5; peroxiredoxin-5, mitochondrial; PLPThioredoxin peroxidase PMP20; PMP20; PRDX5; PRDX6; PRXV; prx-V; SBBI10; Thioredoxin reductase; TPx type VI PRDX5 ACR1, AOEB166, B166, HEL-S-55, PLP, PMP20, PRDX6, PRXV, SBBI10, prx-V peroxiredoxin 5 peroxiredoxin-5, mitochondrial|Alu co-repressor 1|TPx type VI|antioxidant enzyme B166|epididymis secretory protein Li 55|liver tissue 2D-page spot 71B|peroxiredoxin V|peroxisomal antioxidant enzyme|thioredoxin peroxidase PMP20|thioredoxin reductase|thioredoxin-dependent peroxiredoxin 5

For more info on PRDX5, check out the PRDX5 Infographic

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In this infographic, you will see the following information for PRDX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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