Product Info Summary
SKU: | M00871 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-PF4 Monoclonal Antibody
SKU/Catalog Number
M00871
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PF4 Monoclonal Antibody catalog # M00871. Tested in WB, IHC, IP applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PF4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00871)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACED-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PF4 Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00871 is reactive to PF4 in Human, Rat
Applications
M00871 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
15 kDa
Calculated molecular weight
10.845kDa
Background of CXCL4/PF4
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (M00871).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified monoclonal antibody (Catalog # M00871) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 15 kDa. The expected band size for PF4 is at 11 kDa.
Click image to see more details
Western blot analysis of PF4 expression in human spleen lysate.
Protein Target Info & Infographic
Gene/Protein Information For PF4 (Source: Uniprot.org, NCBI)
Gene Name
PF4
Full Name
Platelet factor 4
Weight
10.845kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A PF4 CXCL4, PF-4, SCYB4 platelet factor 4 platelet factor 4|C-X-C motif chemokine 4|chemokine (C-X-C motif) ligand 4|iroplact|oncostatin-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PF4, check out the PF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PF4 Monoclonal Antibody (M00871)
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No publications found for M00871
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PF4 Monoclonal Antibody
Question
Our lab were happy with the WB result of your anti-PF4 Monoclonal antibody. However we have seen positive staining in mononuclear cell secreted. using this antibody. Is that expected? Could you tell me where is PF4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
From literature, mononuclear cell does express PF4. Generally PF4 expresses in secreted. Regarding which tissues have PF4 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 7644496
Boster Scientific Support
Answered: 2020-02-13
Question
I was wanting to use your anti-PF4 Monoclonal antibody for IP for rat leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukocyte identification?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
As indicated on the product datasheet, M00871 anti-PF4 Monoclonal antibody has been tested for IP, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-PF4 Monoclonal antibody M00871. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-03
Question
My question regarding product M00871, anti-PF4 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00871 anti-PF4 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-03
Question
Would anti-PF4 Monoclonal antibody M00871 work for IP with leukocyte?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
According to the expression profile of leukocyte, PF4 is highly expressed in leukocyte. So, it is likely that anti-PF4 Monoclonal antibody M00871 will work for IP with leukocyte.
Boster Scientific Support
Answered: 2019-09-12
Question
Will M00871 anti-PF4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
You can see on the product datasheet, M00871 anti-PF4 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-17
Question
We are currently using anti-PF4 Monoclonal antibody M00871 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The anti-PF4 Monoclonal antibody (M00871) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-13
Question
I see that the anti-PF4 Monoclonal antibody M00871 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-13
Question
My lab would like to test anti-PF4 Monoclonal antibody M00871 on rat leukocyte for research purposes, then I may be interested in using anti-PF4 Monoclonal antibody M00871 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The products we sell, including anti-PF4 Monoclonal antibody M00871, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-23
Question
Is a blocking peptide available for product anti-PF4 Monoclonal antibody (M00871)?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
We do provide the blocking peptide for product anti-PF4 Monoclonal antibody (M00871). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-17
Question
Is this M00871 anti-PF4 Monoclonal antibody reactive to the isotypes of PF4?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
The immunogen of M00871 anti-PF4 Monoclonal antibody is A synthesized peptide derived from human PF4 Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-05
Question
We have tried in the past anti-PF4 Monoclonal antibody for WB on mononuclear cell a few years ago. I am using rat, and We want to use the antibody for IP next. My question regards examining mononuclear cell as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
I looked at the website and datasheets of our anti-PF4 Monoclonal antibody and it appears that M00871 has been tested on rat in both WB and IP. Thus M00871 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-25
Question
See attached the WB image, lot number and protocol we used for leukocyte using anti-PF4 Monoclonal antibody M00871. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-02
Question
We have been able to see staining in rat leukocyte. Do you have any suggestions? Is anti-PF4 Monoclonal antibody supposed to stain leukocyte positively?
G. Jones
Verified customer
Asked: 2015-11-23
Answer
According to literature leukocyte does express PF4. According to Uniprot.org, PF4 is expressed in mononuclear cell, leukocyte, among other tissues. Regarding which tissues have PF4 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 7644496
Boster Scientific Support
Answered: 2015-11-23
Question
Is there a BSA free version of anti-PF4 Monoclonal antibody M00871 available?
R. Rodriguez
Verified customer
Asked: 2013-02-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PF4 Monoclonal antibody M00871 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-02-08