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Product Info Summary
| SKU: | PA1446 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB, ELISA (Cap) |
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Product info
Product Name
Anti-Platelet factor 4 PF4 Antibody Picoband®
SKU/Catalog Number
PA1446
BA4122 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Platelet factor 4 PF4 Antibody catalog # PA1446. Tested in ELISA(Cap), IHC, WB applications. This antibody reacts with Human.The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Platelet factor 4 PF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1446)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PF4.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1446 is reactive to PF4 in Human
Observed Molecular Weight
11 kDa
Calculated molecular weight
10.8 kDa
Background of PF4
CXCL4, Chemokine (C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. CXCL4 belongs to the CXC chemokine family. The CXCL4 gene contains 3 exons spanning approximately 1 kb. The gene is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1446 is guaranteed for IHC, WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA (Cap), 1-5μg/ml, -
Positive Control
WB: recombinant human CXCL4 protein
IHC: human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of CXCL4/PF4 using anti-CXCL4/PF4 antibody (PA1446).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant human CXCL4 protein 5ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL4/PF4 antigen affinity purified polyclonal antibody (PA1446) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CXCL4/PF4 at approximately 11 kDa.
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IHC analysis of CXCL4/PF4 using anti-CXCL4/PF4 antibody (PA1446).
CXCL4/PF4 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL4/PF4 Antibody (PA1446) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Platelet factor 4 PF4 Antibody Picoband® (PA1446)
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5 Customer Q&As for Anti-Platelet factor 4 PF4 Antibody Picoband®
Question
My question regards using your anti-PF4 antibody for negative regulation of megakaryocyte differentiation studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
We appreciate your inquiry. This PA1446 anti-PF4 antibody is tested on lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-05
Question
We purchased anti-PF4 antibody for WB on mononuclear cell a few years ago. I am using human, and We want to use the antibody for IHC next. We want examining mononuclear cell as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
I looked at the website and datasheets of our anti-PF4 antibody and it seems that PA1446 has been validated on human in both WB and IHC. Thus PA1446 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-24
Question
My team were happy with the WB result of your anti-PF4 antibody. However we have been able to see positive staining in leukocyte secreted. using this antibody. Is that expected? Could you tell me where is PF4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-10
Answer
From literature, leukocyte does express PF4. Generally PF4 expresses in secreted. Regarding which tissues have PF4 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 7644496
Boster Scientific Support
Answered: 2018-05-10
Question
We have observed staining in human mononuclear cell. Are there any suggestions? Is anti-PF4 antibody supposed to stain mononuclear cell positively?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
According to literature mononuclear cell does express PF4. According to Uniprot.org, PF4 is expressed in mononuclear cell, leukocyte, among other tissues. Regarding which tissues have PF4 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 7644496
Boster Scientific Support
Answered: 2017-11-20
Question
We are currently using anti-PF4 antibody PA1446 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
C. Huang
Verified customer
Asked: 2015-02-10
Answer
The anti-PF4 antibody (PA1446) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-02-10
