Anti-Platelet factor 4 PF4 Antibody
Rabbit IgG polyclonal antibody for Platelet factor 4(PF4) detection. Tested with WB, IHC-P in Human.
Anti-Platelet factor 4 PF4 Antibody Info At A Glance
CD34 is a single chain transmembrane glycoprotein which is selectively expressed on human lymphoid and myeloid hemapoietic progenitor cells.CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
|Product Name||Anti-Platelet factor 4 PF4 Antibody
See all CXCL4/PF4 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CXCL4/PF4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CXCL4/PF4 information: Molecular Weight: 10845 MW; Subcellular Localization: Secreted.|
|Cite This Product||Anti-Platelet factor 4 PF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1446)|
|Similar Products From
|Anti-Platelet factor 4 PF4 Antibody may replace the following items: sc 19224|sc 23519|sc 26916|sc 367359|sc 374195|sc 398979|sc 50301|sc 57376|sc 57377|sc 71642|sc 73331|sc 73636|sc 73638.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human PF4(85-101aa DLQAPLYKKIIKKLLES).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For PF4 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5196|
|Species Of This Entry||Human|
|Protein Name||Platelet factor 4|
|Superfamily||intercrine alpha (chemokine CxC) family|
|Alternative Names||CXCL4/PF4|chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Nitration; Sulphation; Biotinylation; Glycosylation; Acetylation; Methylation; Carboxylation; Reduction; Amidation; Oxidation|
|Gene Location||4q13.3, on Chromasome 4, gene sequence: NC_000004.12 (73980811..73982124, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For PF4 (Source: Uniprot.org, NCBI)
|Protein Function||Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.|
|Interacting Proteins||APOH; CCL11; CCL13; CCL17; CCL2; CCL20; CCL21; CCL25; CCL26; CCL28; CCL5; CXCL10; CXCL11; CXCL12; CXCL14; CXCL17; CXCL2; CXCL6; CXCL8; CXCL9; XCL1|
|Related Diseases||Heparin-induced Thrombocytopenia; Thrombosis; Heparin-induced Thrombocytopenia (hit); Hemorrhage; Blood Coagulation Disorders; Tissue Adhesions; Neoplasms; Inflammation; Infarction; Myocardial Infarction; Thromboembolism; Malignant Neoplasms; Atherosclerosis; Von Willebrand Disease; Tumor Angiogenesis; Leukemia; Thrombophilia; Venous Thrombosis; Coronary Heart Disease; Diabetes Mellitus|
|Related Pathways||Platelet Activation; Coagulation; Platelet Aggregation; Pathogenesis; Secretion; Angiogenesis; Fibrinolysis; Immune Response; Hemostasis; Cell Proliferation; Blood Coagulation; Localization; Chemotaxis; Complement Activation; Inflammatory Response; Cell Growth; Cell Adhesion; Excretion; Cell Differentiation; Wound Healing|
|Background||CXCL4, Chemokine(C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. CXCL4 belongs to the CXC chemokine family. The CXCL4 gene contains 3 exons spanning approximately 1 kb. The gene is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.|
|Scientific References||PMID: 3098319 by Poncz M., et al. Cloning and characterization of platelet factor 4 cDNA derived from a human erythroleukemic cell line.|
PMID: 1695112 by Eisman R., et al. Structural and functional comparison of the genes for human platelet factor 4 and PF4alt.
Our Boster Quality Guarantee for Anti-Platelet factor 4 PF4 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Recombinant Human CXCL4 Protein 10ng
Lane 2: Recombinant Human CXCL4 Protein 5ng
Lane 3: Recombinant Human CXCL4 Protein 2.5ng
IHC(P): Human Lung Cancer Tissue
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