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- Table of Contents

PPARGC1A antibody (M00236) performs well in WB and shows reactivity with Human, Mouse, Rat.
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
91.0 kDa
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| SKU | M00236 |
|---|---|
| Application | Western Blot |
| Sample | Mouse adipocytes |
| Sample Processing Description | RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | 5% non-fat milk |
| Primary Antibody | PGC1 alpha PPARGC1A Rabbit Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:2000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ABBKINE |
| Results Summary | The image displays Western blot results for the target protein PGC1A and the internal control ACTB across different experimental groups. The target bands are clear and well-defined, showing significant differential expression between the experimental groups; the results are satisfactory. |
Jiaqi Xin, Capital Medical University
Verified customer
Submitted 2026-11-05
We promise Anti-PGC1 alpha PPARGC1A Rabbit Monoclonal Antibody performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.