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Product Info Summary
| SKU: | M02933 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-PGC1 beta PPARGC1B Monoclonal Antibody
SKU/Catalog Number
M02933
BM5484 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PGC1 beta PPARGC1B Monoclonal Antibody catalog # M02933. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PGC1 beta PPARGC1B Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02933)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEOI-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PGC1 beta
Reactive Species
M02933 is reactive to PPARGC1B in Human, Mouse, Rat
Observed Molecular Weight
113 kDa
Calculated molecular weight
113.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02933 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
Positive Control
WB: HeLa cell, Mouse spleen, Rat spleen, human 293T whole cell
Validation Images & Assay Conditions
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Western blot analysis of PPARGC1B using anti-PPARGC1B antibody (M02933).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPARGC1B antigen affinity purified monoclonal antibody (Catalog # M02933) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPARGC1B at approximately 113 kDa. The expected band size for PPARGC1B is at 113 kDa.
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Western blot analysis of PGC1 beta expression in (1) HeLa cell lysate; (2) Mouse spleen lysate; (3) Rat spleen lysate.
Specific Publications For Anti-PGC1 beta PPARGC1B Monoclonal Antibody (M02933)
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2 Customer Q&As for Anti-PGC1 beta PPARGC1B Monoclonal Antibody
Question
We are currently using anti-PGC1 beta Monoclonal antibody M02933 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
The anti-PGC1 beta Monoclonal antibody (M02933) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-16
Question
What sample target on Western Blot application was tested with M02933?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of PGC1 beta expression in (1) HeLa cell lysate; (2) Mouse spleen lysate; (3) Rat spleen lysate.
Boster Scientific Support
Answered: 2020-01-16
