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- Table of Contents
ANP32E antibody (A07456) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of PHAP III in Human Small Intestine cells with PHAP III antibody at 10 μg/mL.
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Anti-PHAP III ANP32E Antibody
ANP32E antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
PHAP III antibody can be used for detection of PHAP III by Western blot at 1 μg/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-PHAP III ANP32E Antibody
View all ANP32E Antibodies
View all ANP32E Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
PHAP III antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
PHAP III Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III. The immunogen is located within amino acids 200 - 250 of PHAP III.
Cross-Reactivity
PHAP III has no cross-reaction to PHAP I and PHAP I2a.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
30.7 kDa
Background of ANP32E
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Cite This Product
Anti-PHAP III ANP32E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07456)
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Product Promise
We promise Anti-PHAP III ANP32E Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A07456
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026