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Product Info Summary
| SKU: | M05822 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PHAPI2 / APRIL Rabbit Monoclonal Antibody
SKU/Catalog Number
M05822
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-PHAPI2 / APRIL Rabbit Monoclonal Antibody catalog # M05822. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHAPI2 / APRIL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05822)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
22A78
Isotype
IgG
Immunogen
A synthesized peptide derived from human PHAPI2 / APRIL
Reactive Species
M05822 is reactive to ANP32B in Human, Mouse, Rat
Observed Molecular Weight
29-32 kDa
Calculated molecular weight
28.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05822 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:100
Positive Control
WB: human Jurkat whole cell, human LNCAP whole cell, human U2OS whole cell, human A431 whole cell, human PC-3 whole cell, human MCF-7 whole cell, rat C6 whole cell, mouse RAW264.7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of ANP32B using anti-ANP32B antibody (M05822).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human LNCAP whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human MCF-7 whole cell lysates,
Lane 7: rat C6 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANP32B antigen affinity purified monoclonal antibody (M05822) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ANP32B at approximately 29-32 kDa. The expected band size for ANP32B is at 29 kDa.
Specific Publications For Anti-PHAPI2 / APRIL Rabbit Monoclonal Antibody (M05822)
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