Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody

Product Name

Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody

View all EGLN1/PHD2 Antibodies

SKU/Catalog Number

M00415

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody catalog # M00415. Tested in WB, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00415)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACEF-5

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PHD2 / prolyl hydroxylase Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00415 is reactive to EGLN1 in Human

Applications

M00415 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

46.021kDa

Background of EGLN1/PHD2

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

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Western blot analysis of PHD2 / prolyl hydroxylase expression in SH-SY5Y cell lysate.

Gene/Protein Information For EGLN1 (Source: Uniprot.org, NCBI)

Alternative Names

C1orf12; C1orf12HPH2; EC 1.14.11; EC 1.14.11.-; ECYT3; egl nine homolog 1 (C. elegans); egl nine homolog 1; egl nine-like protein 1; EGLN1; HIF prolyl hydroxylase 2; HIFPH2 EGL nine (C.elegans) homolog 1; HIFPH2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH2; HPH-2; Hypoxia-inducible factor prolyl hydroxylase 2; PHD2; PHD2SM20; Prolyl hydroxylase domain-containing protein 2; SM20; SM-20; zinc finger MYND domain-containing protein 6; ZMYND6; ZMYND6DKFZp761F179 EGLN1 C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6 egl-9 family hypoxia inducible factor 1 egl nine homolog 1|HIF-prolyl hydroxylase 2|egl nine-like protein 1|hypoxia-inducible factor prolyl hydroxylase 2|prolyl hydroxylase domain-containing protein 2|zinc finger MYND domain-containing protein 6

For more info on EGLN1, check out the EGLN1 Infographic

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In this infographic, you will see the following information for EGLN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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