Click image to see more details
Product Info Summary
| SKU: | A10188-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-PHD finger protein 3 PHF3 Antibody
SKU/Catalog Number
A10188-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PHD finger protein 3 PHF3 Antibody catalog # A10188-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHD finger protein 3 PHF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10188-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PHF3. AA range:1990-2039
Reactive Species
A10188-1 is reactive to PHF3 in Human, Mouse
Calculated molecular weight
229.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10188-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using PHF3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PHF3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-PHD finger protein 3 PHF3 Antibody (A10188-1)
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Customer Q&As
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5 Customer Q&As for Anti-PHD finger protein 3 PHF3 Antibody
Question
Is this A10188-1 anti-PHF3 antibody reactive to the isotypes of PHF3?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
The immunogen of A10188-1 anti-PHF3 antibody is Synthesized peptide derived from the C-terminal region of human PHF3. at AA rangle: 1970-2050. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-17
Question
Is a blocking peptide available for product anti-PHF3 antibody (A10188-1)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
We do provide the blocking peptide for product anti-PHF3 antibody (A10188-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-02
Question
Do you have a BSA free version of anti-PHF3 antibody A10188-1 available?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PHF3 antibody A10188-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-30
Question
Will A10188-1 anti-PHF3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Jha
Verified customer
Asked: 2019-05-17
Answer
As indicated on the product datasheet, A10188-1 anti-PHF3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-17
Question
We are currently using anti-PHF3 antibody A10188-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-19
Answer
The anti-PHF3 antibody (A10188-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-19
