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Product Info Summary
| SKU: | A02430-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-PHLPP1 Antibody
SKU/Catalog Number
A02430-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PHLPP1 Antibody (Catalog # A02430-2). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
PHLPP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PHLPP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02430-2)
Host
Rabbit
Contents
PHLPP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1. The immunogen is located within amino acids 250 - 300 of PHLPP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.
Reactive Species
A02430-2 is reactive to PHLPP1 in Human
Applications
A02430-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
184.672kDa
Background of PHLPP
PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PHLPP1 antibody can be used for detection of PHLPP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PHLPP1 in SW480 cell lysate with PHLPP1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of PHLPP1 in SW480 cells with PHLPP1 antibody at 2.5 μg/ml.
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Immunofluorescence of PHLPP1 in SW480 cells with PHLPP1 antibody at 5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For PHLPP1 (Source: Uniprot.org, NCBI)
Gene Name
PHLPP1
Full Name
PH domain leucine-rich repeat-containing protein phosphatase 1
Weight
184.672kDa
Alternative Names
EC 3.1.3.16; hSCOP; KIAA0606; MGC161555; PH domain and leucine rich repeat protein phosphatase 1; PH domain and leucine rich repeat protein phosphatase; PH domain leucine-rich repeat-containing protein phosphatase 1; PHLPP; pleckstrin homology domain containing, family E (with leucine rich repeats)member 1; Pleckstrin homology domain-containing family E member 1; PLEKHE1; SCN circadian oscillatory protein; SCOPPH domain-containing family E member 1; Suprachiasmatic nucleus circadian oscillatory protein PHLPP1 PHLPP, PLEKHE1, PPM3A, SCOP PH domain and leucine rich repeat protein phosphatase 1 PH domain leucine-rich repeat-containing protein phosphatase 1|PH domain-containing family E member 1|SCN circadian oscillatory protein|pleckstrin homology domain containing, family E (with leucine rich repeats) member 1|protein phosphatase, Mg2+/Mn2+ dependent 3A|suprachiasmatic nucleus circadian oscillatory protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHLPP1, check out the PHLPP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHLPP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHLPP1 Antibody (A02430-2)
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Customer Reviews
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