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Product Info Summary
| SKU: | A01183-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PHO1/APOBEC3A Antibody Picoband®
SKU/Catalog Number
A01183-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PHO1/APOBEC3A Antibody Picoband® catalog # A01183-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHO1/APOBEC3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01183-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PHO1 recombinant protein (Position: M1-L63).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01183-1 is reactive to APOBEC3A in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
23.0 kDa
Background of APOBEC3A
Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, also known as APOBEC3A, is a gene of the APOBEC3 family found in humans, non-human primates, and some other mammals.This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01183-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human CACO-2 whole cell, human A549 whole cell, rat PC-12 whole cell, mouse RAW264.7 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of APOBEC3A using anti-APOBEC3A antibody (A01183-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3A antigen affinity purified polyclonal antibody (Catalog # A01183-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3A at approximately 23 kDa. The expected band size for APOBEC3A is at 23 kDa.
Specific Publications For Anti-PHO1/APOBEC3A Antibody Picoband® (A01183-1)
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7 Customer Q&As for Anti-PHO1/APOBEC3A Antibody Picoband®
Question
Does anti-PHO1/APOBEC3A antibody A01183-1 work on primate ELISA with brain?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
Our lab technicians have not tested anti-PHO1/APOBEC3A antibody A01183-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PHO1/APOBEC3A antibody A01183-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-25
Question
Is this A01183-1 anti-PHO1/APOBEC3A antibody reactive to the isotypes of APOBEC3A?
B. Jackson
Verified customer
Asked: 2018-07-27
Answer
The immunogen of A01183-1 anti-PHO1/APOBEC3A antibody is E. coli-derived human PHO1 recombinant protein (Position: M1-L63). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-27
Question
I was wanting to use your anti-PHO1/APOBEC3A antibody for ELISA for mouse keratinocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse keratinocyte identification?
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
It shows on the product datasheet, A01183-1 anti-PHO1/APOBEC3A antibody has been tested for ELISA, Flow Cytometry, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse keratinocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-10
Question
Does anti-PHO1/APOBEC3A antibody A01183-1 work for ELISA with keratinocyte?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
According to the expression profile of keratinocyte, APOBEC3A is highly expressed in keratinocyte. So, it is likely that anti-PHO1/APOBEC3A antibody A01183-1 will work for ELISA with keratinocyte.
Boster Scientific Support
Answered: 2018-03-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for keratinocyte using anti-PHO1/APOBEC3A antibody A01183-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-25
Question
We are currently using anti-PHO1/APOBEC3A antibody A01183-1 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
G. Huang
Verified customer
Asked: 2017-07-04
Answer
The anti-PHO1/APOBEC3A antibody (A01183-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-04
Question
We want to test anti-PHO1/APOBEC3A antibody A01183-1 on mouse keratinocyte for research purposes, then I may be interested in using anti-PHO1/APOBEC3A antibody A01183-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Brown
Verified customer
Asked: 2013-10-28
Answer
The products we sell, including anti-PHO1/APOBEC3A antibody A01183-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-28
