Anti-PHO1/APOBEC3A Antibody Picoband™

Product Name

Anti-PHO1/APOBEC3A Antibody Picoband™

View all APOBEC3A Antibodies

SKU/Catalog Number

A01183-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PHO1/APOBEC3A Antibody Picoband™ catalog # A01183-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PHO1/APOBEC3A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01183-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human PHO1 recombinant protein (Position: M1-L63).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01183-1 is reactive to APOBEC3A in Human, Mouse

Applications

A01183-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

23.012kDa

Background of APOBEC3A

Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, also known as APOBEC3A, is a gene of the APOBEC3 family found in humans, non-human primates, and some other mammals.This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PHO1 using anti-PHO1 antibody (A01183-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: mouse SP20 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHO1 antigen affinity purified polyclonal antibody (Catalog # A01183-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHO1 at approximately 28KD. The expected band size for PHO1 is at 23KD.

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Figure 2. Flow Cytometry analysis of A549 cells using anti-PHO1 antibody (A01183-1).
Overlay histogram showing A549 cells stained with A01183-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHO1 Antibody (A01183-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For APOBEC3A (Source: Uniprot.org, NCBI)

Alternative Names

apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A; ARP3; bK150C2.1; EC 3.5.4; EC 3.5.4.-; phorbolin 1; phorbolin-1; PHRBNprobable DNA dC->dU-editing enzyme APOBEC-3A APOBEC3A A3A, ARP3, PHRBN, bK150C2.1 apolipoprotein B mRNA editing enzyme catalytic subunit 3A DNA dC->dU-editing enzyme APOBEC-3A|apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A|phorbolin-1|probable DNA dC->dU-editing enzyme APOBEC-3A

For more info on APOBEC3A, check out the APOBEC3A Infographic

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In this infographic, you will see the following information for APOBEC3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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