Anti-Phospho-ABL1/2 (Tyr393/439) Antibody

Product Name

Anti-Phospho-ABL1/2 (Tyr393/439) Antibody

View all c-Abl Antibodies

SKU/Catalog Number

P00133

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-ABL1/2 (Tyr393/439) Antibody (Catalog # P00133). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-ABL1/2 (Tyr393/439) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00133)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around phosphorylation site of tyrosine 393/439 (D-T-Y(p)-T-A) derived from Human ABL1/2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P00133 is reactive to ABL1 in Human, Mouse

Applications

P00133 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

122.873kDa

Background of c-Abl

Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.

Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
Pluk, H. et al. (2002) Cell 108, 247-259.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 210kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2(Phospho-Tyr393/439) antibody P00133(left) or the same antibody preincubated with blocking peptide(right).

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Western blot analysis of extracts from HL60 cells using ABL1/2(phospho-Tyr393/439) antibody P00133 and the same antibody preincubated with blocking peptide.

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Immunofluorescence staining of methanol-fixed Hela cells using ABL1/2(phospho-Tyr393/439) antibody P00133.

Gene/Protein Information For ABL1 (Source: Uniprot.org, NCBI)

Alternative Names

Abelson murine leukemia viral oncogene homolog 1; ABL; ABL1; bcr/abl; BCR-ABL1; c-abl oncogene 1, non-receptor tyrosine kinase; c-abl oncogene 1, receptor tyrosine kinase; cAbl; c-Abl; EC 2.7.10; EC 2.7.10.2; JTK7; JTK7bcr/abl; p150bcr/c-abl oncogene protein; Proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolog 1; v-abl ABL1 ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl ABL proto-oncogene 1, non-receptor tyrosine kinase tyrosine-protein kinase ABL1|ABL protooncogene 1 nonreceptor tyrosine kinase|Abelson tyrosine-protein kinase 1|bcr/c-abl oncogene protein|c-abl oncogene 1, receptor tyrosine kinase|proto-oncogene c-Abl|proto-oncogene tyrosine-protein kinase ABL1|truncated ABL protooncogene 1 nonreceptor tyrosine kinase|v-abl Abelson murine leukemia viral oncogene homolog 1

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