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- Table of Contents
PRKAA1 antibody (P00994-1) performs well in IP, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Western blot analysis of Phospho-AMPK alpha 1 (S496) expression in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.
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Anti-Phospho-AMPK alpha 1 (S496) PRKAA1 Rabbit Monoclonal Antibody
PRKAA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IP, IF, ICC, WB |
Tested Applications
PRKAA1 antibody was positively detected by WB in:
293T cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Phospho-AMPK alpha 1 (S496) PRKAA1 Rabbit Monoclonal Antibody
View all PRKAA1 Antibodies
View all PRKAA1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HDH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AMPK alpha 1
Observed Molecular Weight
64 kDa
Calculated Molecular Weight
64.0 kDa
Cite This Product
Anti-Phospho-AMPK alpha 1 (S496) PRKAA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00994-1)
Protocols
Western Blot protocol for PRKAA1 antibody
Publications
Application:
Species:
Unraveling the role of abnormal AMPK and CRMP-2 phosphorylation in developmental fluoride neurotoxicity: Implications for synaptic damage and neurological disorders
Published: 2025-04-15
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 6.10
Species: Rat
PMID: 40239547
Sigma-1 Receptor Rescues Autophagy Through AMPK/mTOR Signaling Pathway in Sepsis-Induced Acute Kidney Injury
Published: 2025-04-03
Journal: International Journal of General Medicine
Application: WB
Impact Factor: 2.00
Species: Human
PMID: 40201929
Function of AMPK/mTOR Signaling in TGF-β1-Induced Pterygium Fibroblast Proliferation and Transdifferentiation
Published: 2025-02-23
Journal: CURRENT EYE RESEARCH
Application: WB
Impact Factor: 2.00
Species: Human
PMID: 39988428
Anti-fatigue effects and mechanism of Syngnathus schlegeli peptides supplementation on exercise-fatigued mice
Published: 2023-10-17
Journal: Journal of Functional Foods
Application: WB
Impact Factor: 5.60
Species: Mouse
The Function behind the Relation between Lipid Metabolism and Vimentin on H9N2 Subtype AIV Replication
Published: 2022-08-18
Journal: Viruses-Basel
Application: WB
Impact Factor: 5.82
Species: Human
PMID: 36016436
Fructus Zanthoxyli extract improves glycolipid metabolism disorder of type 2 diabetes mellitus via activation of AMPK/PI3K/Akt pathway: Network pharmacology and experimental validation
Published: 2022-07-26
Journal: Journal of Integrative Medicine-JIM
Application: WB,IF
Impact Factor: 3.95
Species: Human
PMID: 35965234
The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway
Published: 2022-07-04
Journal: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 2.34
Species: Mouse
PMID: 35786714
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro
Published: 2019-07-05
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: WB
Impact Factor: 3.59
Species: Human
PMID: 31283929
Role of Phosphorylated AMP-Activated Protein Kinase (AMPK) in Myocardial Insulin Resistance After Myocardial Ischemia-Reperfusion During Cardiopulmonary Bypass Surgery in Dogs
Published: 2019-06-04
Journal: MEDICAL SCIENCE MONITOR
Application: IHC
Impact Factor: 1.98
Species: Dog
PMID: 31160548
Curcumin inhibits aerobic glycolysis in hepatic stellate cells associated with activation of adenosine monophosphate-activated protein kinase
Published: 2016-06-09
Journal: IUBMB LIFE
Application: IF
Impact Factor: 2.65
Species: Rat
PMID: 27278959
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Product Promise
We promise Anti-Phospho-AMPK alpha 1 (S496) PRKAA1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00994-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026