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Product Info Summary
| SKU: | MP03520 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-Bad (S112) Monoclonal Antibody
SKU/Catalog Number
MP03520
BM5507 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Bad (S112) Monoclonal Antibody catalog # MP03520. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Bad (S112) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP03520)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEBH-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bad Promotes cell death. Successfully competes for the binding to Bcl-X (L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX.
Reactive Species
MP03520 is reactive to BAD in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
18.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP03520 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Positive Control
WB: HeLa cell, HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Bad (S112) expression in (1) HeLa cell lysate; (2) HeLa cell treated with Calcyculin A lysate.
Specific Publications For Anti-Phospho-Bad (S112) Monoclonal Antibody (MP03520)
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5 Customer Q&As for Anti-Phospho-Bad (S112) Monoclonal Antibody
Question
My question regarding product MP03520, anti-Phospho-Bad (S112) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MP03520 anti-Phospho-Bad (S112) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-29
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Phospho-Bad (S112) Monoclonal antibody MP03520. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-10
Question
We are currently using anti-Phospho-Bad (S112) Monoclonal antibody MP03520 for mouse tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
The anti-Phospho-Bad (S112) Monoclonal antibody (MP03520) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-07
Question
Will anti-Phospho-Bad (S112) Monoclonal antibody MP03520 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
According to the expression profile of cervix carcinoma erythroleukemia, BAD is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Phospho-Bad (S112) Monoclonal antibody MP03520 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2017-07-21
Question
Our lab want to know about to test anti-Phospho-Bad (S112) Monoclonal antibody MP03520 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Phospho-Bad (S112) Monoclonal antibody MP03520 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Edwards
Verified customer
Asked: 2013-08-26
Answer
The products we sell, including anti-Phospho-Bad (S112) Monoclonal antibody MP03520, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-08-26
