Click image to see more details
Product Info Summary
| SKU: | P00245 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-BTK (Y551) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00245
BM4567 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-BTK (Y551) Rabbit Monoclonal Antibody catalog # P00245. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-BTK (Y551) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00245)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FII-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-BTK (Y551)
Reactive Species
P00245 is reactive to BTK in Human
Observed Molecular Weight
74 kDa
Calculated molecular weight
76.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00245 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: Ramos cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-BTK (Y551) expression in Ramos cell lysate treated with Pervanadate.
Specific Publications For Anti-Phospho-BTK (Y551) Rabbit Monoclonal Antibody (P00245)
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3 Customer Q&As for Anti-Phospho-BTK (Y551) Rabbit Monoclonal Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Phospho-BTK (Y551) Rabbit Monoclonal antibody P00245. Let me know if you need anything else.
G. Roberts
Verified customer
Asked: 2020-03-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-16
Question
Is this P00245 anti-Phospho-BTK (Y551) Rabbit Monoclonal antibody reactive to the isotypes of BTK?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The immunogen of P00245 anti-Phospho-BTK (Y551) Rabbit Monoclonal antibody is A synthesized peptide derived from human Phospho-BTK (Y551). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-30
Question
I was wanting to use your anti-Phospho-BTK (Y551) Rabbit Monoclonal antibody for IF for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
You can see on the product datasheet, P00245 anti-Phospho-BTK (Y551) Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-05
