Anti-Phospho-Casein Kinase alpha (Y294) CSNK1A1 Antibody

Product Name

Anti-Phospho-Casein Kinase alpha (Y294) CSNK1A1 Antibody

View all Casein Kinase 1 alpha Antibodies

SKU/Catalog Number

A07171Y294

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-Casein Kinase alpha (Y294) CSNK1A1 Antibody catalog # A07171Y294. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Casein Kinase alpha (Y294) CSNK1A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07171Y294)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Casein Kinase Ialpha around the phosphorylation site of Y294.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07171Y294 is reactive to CSNK1A1 in Human, Mouse, Rat

Applications

A07171Y294 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

38.915kDa

Background of Casein Kinase 1 alpha

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10, its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Alternate splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Herzog H.; Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
Wang Q., Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Yoshihama M., Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of CSNK1A1 using Anti-Phospho-Casein Kinase alpha (Y294) CSNK1A1 Antibody (A07171Y294).

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C

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Figure 1. Western blotting validation for Anti-Phospho-Casein Kinase alpha (Y294) CSNK1A1 Antibody A07171Y294

Western Blot (WB) analysis of HBE cells using Phospho-Casein Kinase Ialpha (Y294) polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For CSNK1A1 (Source: Uniprot.org, NCBI)

Alternative Names

Casein Kinase 1 alpha; casein kinase 1, alpha 1; casein kinase I isoform alpha; CK1CKI-alpha; CSNK1A1; down-regulated in lung cancer; EC 2.7.11.1; HLCDGP1; PRO2975 CSNK1A1 CK1, CK1a, CKIa, HEL-S-77p, HLCDGP1, PRO2975 casein kinase 1 alpha 1 casein kinase I isoform alpha|CKI-alpha|clock regulator kinase|down-regulated in lung cancer|epididymis secretory sperm binding protein Li 77p

For more info on CSNK1A1, check out the CSNK1A1 Infographic

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In this infographic, you will see the following information for CSNK1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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