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Product Info Summary
| SKU: | A02398Y255 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Casein Kinase I alpha (Y255) CSNK2A1 Antibody
SKU/Catalog Number
A02398Y255
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Casein Kinase I alpha (Y255) CSNK2A1 Antibody catalog # A02398Y255. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Casein Kinase I alpha (Y255) CSNK2A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02398Y255)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Casein Kinase II alpha around the phosphorylation site of Tyr255. AA range:221-270
Reactive Species
A02398Y255 is reactive to CSNK2A1 in Human, Mouse, Rat
Calculated molecular weight
45.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02398Y255 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of HeLa cells using Phospho-Casein Kinase IIα (Y255) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from HeLa cells and Jurkat cells, using Casein Kinase II alpha (Phospho-Tyr255) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Casein Kinase II alpha (Phospho-Tyr255) Antibody
Specific Publications For Anti-Phospho-Casein Kinase I alpha (Y255) CSNK2A1 Antibody (A02398Y255)
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