Anti-Phospho-Cdc25B (S353) Antibody

Product Name

Anti-Phospho-Cdc25B (S353) Antibody

View all CDC25B Antibodies

SKU/Catalog Number

A01899S353

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-Cdc25B (S353) Antibody catalog # A01899S353. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Cdc25B (S353) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01899S353)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Cdc25B around the phosphorylation site of S353.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01899S353 is reactive to CDC25B in Human, Monkey

Applications

A01899S353 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

64.987kDa

Background of CDC25B

Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions By similarity. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.

Joberty G., Nat. Cell Biol. 2:531-539(2000).
Fang C.M., Cell Res. 11:223-229(2001).
Kohjima M., Biochem. Biophys. Res. Commun. 299:641-646(2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For CDC25B (Source: Uniprot.org, NCBI)

Alternative Names

CDC25B; CDC25HU2; cell division cycle 25 homolog B (S. cerevisiae); cell division cycle 25 homolog B (S. pombe); cell division cycle 25B; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2 CDC25B cell division cycle 25B M-phase inducer phosphatase 2|CDC25 homolog B|dual specificity phosphatase Cdc25B

For more info on CDC25B, check out the CDC25B Infographic

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In this infographic, you will see the following information for CDC25B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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