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Product Info Summary
| SKU: | MP00166 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody
SKU/Catalog Number
MP00166
BM4590 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody catalog # MP00166. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00166)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GBB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDK2.
Reactive Species
MP00166 is reactive to CDK2 in Human, Mouse, Rat
Observed Molecular Weight
34 kDa
Calculated molecular weight
33.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP00166 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human K562 whole cell, human A431 whole cell, human 293T whole cell, mouse Neuro-2a whole cell
IHC: human bladder cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of P-CDK2 using anti-P-CDK2 antibody (MP00166).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-CDK2 antigen affinity purified monoclonal antibody (MP00166) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-CDK2 at approximately 34 kDa. The expected band size for P-CDK2 is at 34 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-CDK2 (Y15) Antibody.
Specific Publications For Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody (MP00166)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody
Question
I am interested in to test anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The products we sell, including anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-12
Question
Is a blocking peptide available for product anti-Phospho-CDK2 (Y15) Monoclonal antibody (MP00166)?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
We do provide the blocking peptide for product anti-Phospho-CDK2 (Y15) Monoclonal antibody (MP00166). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-15
Question
We are currently using anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The anti-Phospho-CDK2 (Y15) Monoclonal antibody (MP00166) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-27
Question
Would MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
It shows on the product datasheet, MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-26
Question
Our lab used your anti-Phospho-CDK2 (Y15) Monoclonal antibody for IP on cervix carcinoma a few years ago. I am using rat, and We intend to use the antibody for IHC next. We are interested in examining cervix carcinoma as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
I have checked the website and datasheets of our anti-Phospho-CDK2 (Y15) Monoclonal antibody and it appears that MP00166 has been validated on rat in both IP and IHC. Thus MP00166 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-22
Question
Our lab were well pleased with the WB result of your anti-Phospho-CDK2 (Y15) Monoclonal antibody. However we have been able to see positive staining in left lobe of thyroid gland cytoskeleton using this antibody. Is that expected? Could you tell me where is CDK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
Based on literature, left lobe of thyroid gland does express CDK2. Generally CDK2 expresses in cytoplasm, cytoskeleton, microtubule. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-04-12
Question
Is there a BSA free version of anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 available?
N. Wu
Verified customer
Asked: 2018-05-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-28
Question
Is this MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody reactive to the isotypes of CDK2?
Verified Customer
Verified customer
Asked: 2017-12-22
Answer
The immunogen of MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody is A synthesized peptide derived from human CDK2 CDK2 is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. This protein can be regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-22
Question
Will anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 work for WB with leukemic t-cell?
W. Collins
Verified customer
Asked: 2017-06-06
Answer
According to the expression profile of leukemic t-cell, CDK2 is highly expressed in leukemic t-cell. So, it is likely that anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2017-06-06
Question
We have seen staining in mouse left lobe of thyroid gland. Any tips? Is anti-Phospho-CDK2 (Y15) Monoclonal antibody supposed to stain left lobe of thyroid gland positively?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
Based on literature left lobe of thyroid gland does express CDK2. Based on Uniprot.org, CDK2 is expressed in left lobe of thyroid gland, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-26
Question
I have attached the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166. Please let me know if you require anything else.
A. Anderson
Verified customer
Asked: 2017-02-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-01
Question
I was wanting to use your anti-Phospho-CDK2 (Y15) Monoclonal antibody for WB for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?
A. Yang
Verified customer
Asked: 2015-11-25
Answer
As indicated on the product datasheet, MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody has been validated for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-25
Question
I see that the anti-Phospho-CDK2 (Y15) Monoclonal antibody MP00166 works with WB, what is the protocol used to produce the result images on the product page?
B. Walker
Verified customer
Asked: 2015-06-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-06-22
Question
I have a question about product MP00166, anti-Phospho-CDK2 (Y15) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Krishna
Verified customer
Asked: 2014-04-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MP00166 anti-Phospho-CDK2 (Y15) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-04-28
