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- Table of Contents
CHEK2 antibody (P00277-1) performs well in IP, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of Phospho-Chk2 (T68) expression in (1) 293 treated with UV and Untreated cell lysate; (2) Untreated.
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Anti-Phospho-Chk2 (T68) Rabbit Monoclonal Antibody
CHEK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IP, WB |
Tested Applications
CHEK2 antibody was positively detected by WB in:
293 cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Phospho-Chk2 (T68) Rabbit Monoclonal Antibody
View all CHEK2 Antibodies
View all CHEK2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
27C74
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-Chk2 (T68)
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
60.9 kDa
Cite This Product
Anti-Phospho-Chk2 (T68) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00277-1)
Protocols
Western Blot protocol for CHEK2 antibody
Review
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1 Review For Anti-Phospho-Chk2 (T68) Rabbit Monoclonal Antibody
Works very well in Western blot for detecting pChk2 in human HeLa cells.
| SKU | P00277-1 |
|---|---|
| Application | Western Blot |
| Sample | Hela cell |
| Sample Processing Description | After 12 h of viral infection, cells were lysed in RIPA buffer with PMSF and heated in 1× protein loading buffer for 10 min to denature proteins. |
| Primary Incubation | The membrane was incubated with the CHEK2 (Phospho-T68) primary antibody (1:2000) overnight at 3 hours. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | None |
| Detection | Signal was developed using ECL substrate on an ChemiDoc MP system. |
| Results Summary | Works very well in Western blot for detecting pChk2 in human HeLa cells. |
Jiawei Liao, China Agricultural University
Verified customer
Submitted 2025-09-23
Product Promise
We promise Anti-Phospho-Chk2 (T68) Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00277-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026