Click image to see more details
Product Info Summary
| SKU: | P06400 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-Phospho CNOT2 (Ser101) Antibody
SKU/Catalog Number
P06400
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho CNOT2 (Ser101) Antibody (Catalog# P06400). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho CNOT2 (Ser101) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06400)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CNOT2 around the phosphorylation site of Ser101. Immunogen sequence location: 67-116
Reactive Species
P06400 is reactive to CNOT2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
59.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P06400 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CNOT2 (Phospho-Ser101) Antibody
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain, using CNOT2 (Phospho-Ser101) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho CNOT2 (Ser101) Antibody (P06400)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho CNOT2 (Ser101) Antibody
Question
Is there a BSA free version of anti-Phospho CNOT2 (Ser101) antibody P06400 available?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho CNOT2 (Ser101) antibody P06400 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-04
Question
We are currently using anti-Phospho CNOT2 (Ser101) antibody P06400 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Phospho CNOT2 (Ser101) antibody (P06400) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
Question
Does anti-Phospho CNOT2 (Ser101) antibody P06400 work for IHC with heart?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
According to the expression profile of heart, CNOT2 is highly expressed in heart. So, it is likely that anti-Phospho CNOT2 (Ser101) antibody P06400 will work for IHC with heart.
Boster Scientific Support
Answered: 2019-02-18
Question
I see that the anti-Phospho CNOT2 (Ser101) antibody P06400 works with IHC, what is the protocol used to produce the result images on the product page?
C. Zhang
Verified customer
Asked: 2016-02-18
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-02-18
