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Product Info Summary
| SKU: | M02333Y228 |
|---|---|
| Size: | 100 µl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-CTNND1 (Y228) Rabbit Monoclonal Antibody
SKU/Catalog Number
M02333Y228
Size
100 µl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-CTNND1 (Y228) Rabbit Monoclonal Antibody catalog # M02333Y228. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-CTNND1 (Y228) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02333Y228)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
32C33
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-CTNND1 (Y228)
Reactive Species
M02333Y228 is reactive to CTNND1 in Human, Mouse, Rat
Observed Molecular Weight
90, 115 kDa
Calculated molecular weight
108.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02333Y228 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human A431 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-CTNND1 using anti-Phospho-CTNND1 antibody (M02333Y228).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions.
Lane 1: A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-CTNND1 antigen affinity purified monoclonal antibody (M02333Y228) at 0.5 μg/ml overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. The expected band size for ANO1 is at 108 kDa.
Specific Publications For Anti-Phospho-CTNND1 (Y228) Rabbit Monoclonal Antibody (M02333Y228)
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