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- Table of Contents
MAPK3 antibody (P00104-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Western blot analysis of Phospho- Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF.
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Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) MAPK3 Rabbit Monoclonal Antibody
MAPK3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
MAPK3 antibody was positively detected by WB in:
A431 cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:40
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
ICC
IF
FC
Human
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Product Information
Product Name
Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) MAPK3 Rabbit Monoclonal Antibody
View all MAPK3 Antibodies
View all MAPK3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IH-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187)
Observed Molecular Weight
43, 41 kDa
Calculated Molecular Weight
43.1 kDa
Cite This Product
Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) MAPK3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00104-1)
Protocols
Western Blot protocol for MAPK3 antibody
Publications
Application:
Species:
Synergistic Antitumor Activity and Neuroprotective Effects of FGF1/FGFR Inhibition with Oxaliplatin Chemotherapy
Published: 2026-02-10
Journal: Journal of Advanced Research
Application: WB
Impact Factor: 13.00
Species: Human
PMID: 41672244
Synergistic therapy for diabetic retinopathy via dual blockade of VEGF-A and TNF-α with a bispecific nanobody
Published: 2026-01-06
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.80
Species: Human
PMID: 41567638
Structural characteristics and immunomodulation activity of a polysaccharide from purslane (Portulaca oleracea)
Published: 2023-09-16
Journal: Journal of Functional Foods
Application: WB
Impact Factor: 5.60
Species: Mouse
Dioscin induces osteosarcoma cell apoptosis by upregulating ROS-mediated P38 MAPK signaling
Published: 2022-11-19
Journal: DRUG DEVELOPMENT RESEARCH
Application: WB
Impact Factor: 5.00
Species: Human
PMID: 36401839
KISS-1, Mediated by Promoter Methylation, Suppresses Esophageal Squamous Cell Carcinoma Metastasis via MMP2/9/MAPK Axis
Published: 2022-01-07
Journal: DIGESTIVE DISEASES AND SCIENCES
Application: WB
Impact Factor: 3.49
Species: Human
PMID: 34993679
Morin alleviates LPS-induced mastitis by inhibiting the PI3K/AKT, MAPK, NF-κB and NLRP3 signaling pathway and protecting the integrity of blood-milk barrier
Published: 2019-11-08
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 3.36
Species: Mouse
PMID: 31711938
Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro
Published: 2019-10-28
Journal: CELL BIOCHEMISTRY AND BIOPHYSICS
Application: WB
Impact Factor: 2.32
Species: Human
PMID: 31659617
Combined use of vitamin E and nimodipine ameliorates dibutyl phthalate-induced memory deficit and apoptosis in mice by inhibiting the ERK 1/2 pathway
Published: 2019-02-15
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: IHC
Impact Factor: 3.59
Species: Mouse
PMID: 30776390
Effect of inhibition of the adrenomedullin gene on the growth and chemosensitivity of ovarian cancer cells
Published: 2012-01-26
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 1.84
Species: Human
PMID: 22294191
High-Expression of Transforming Growth Factor β1 and Phosphorylation of Extracellular Signal-Regulated Protein Kinase in Vascular Smooth Muscle Cells from Aorta and Renal Arterioles of Spontaneous Hypertension Rats
Published: 2009-07-03
Journal: CLINICAL AND EXPERIMENTAL HYPERTENSION
Application: IHC,WB
Impact Factor: 1.08
Species: Rat
PMID: 17364610
Review
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Product Promise
We promise Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) MAPK3 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
P00104-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 16, 2026