Product Info Summary
| SKU: | P03282 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-Phospho Girdin (Ser1417) CCDC88A Antibody
SKU/Catalog Number
P03282
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho Girdin (Ser1417) CCDC88A Antibody (Catalog# P03282). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho Girdin (Ser1417) CCDC88A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03282)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Girdin around the phosphorylation site of Ser1417. Immunogen sequence location: 1383-1432
Reactive Species
P03282 is reactive to CCDC88A in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
216.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03282 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:5000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Girdin (Phospho-Ser1417) Antibody
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Girdin (Phospho-Ser1417) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho Girdin (Ser1417) CCDC88A Antibody (P03282)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho Girdin (Ser1417) CCDC88A Antibody
Question
Is a blocking peptide available for product anti-Phospho Girdin (Ser1417) antibody (P03282)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
We do provide the blocking peptide for product anti-Phospho Girdin (Ser1417) antibody (P03282). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-16
Question
Is this P03282 anti-Phospho Girdin (Ser1417) antibody reactive to the isotypes of CCDC88A?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The immunogen of P03282 anti-Phospho Girdin (Ser1417) antibody is A peptide derived from human Girdin around the phosphorylation site of Ser1417. Immunogen sequence location: 1383-1432. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-15
Question
We are currently using anti-Phospho Girdin (Ser1417) antibody P03282 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
The anti-Phospho Girdin (Ser1417) antibody (P03282) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-26
Question
Can you help my question with product P03282, anti-Phospho Girdin (Ser1417) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Li
Verified customer
Asked: 2017-06-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P03282 anti-Phospho Girdin (Ser1417) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-12

