Product Info Summary
SKU: | A03051S21 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC |
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Product info
Product Name
Anti-Phospho-HMG-14 (S21) HMGN1 Antibody
View all HMGN1/HMG14 Antibodies
SKU/Catalog Number
A03051S21
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-HMG-14 (S21) HMGN1 Antibody catalog # A03051S21. Tested in ELISA, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-HMG-14 (S21) HMGN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03051S21)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human HMG-14 around the phosphorylation site of S21.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03051S21 is reactive to HMGN1 in Human, Mouse, Rat
Applications
A03051S21 is guaranteed for ELISA, IF, IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
10.659kDa
Background of HMGN1/HMG14
Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.
Lemercier C., Mol. Cell. Biol. 20:6627-6637(2000).
Hillier L.W., Nature 424:157-164(2003).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunohistochemistry validation of HMGN1 using Anti-Phospho-HMG-14 (S21) HMGN1 Antibody (A03051S21).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°CProtein Target Info & Infographic
Gene/Protein Information For HMGN1 (Source: Uniprot.org, NCBI)
Gene Name
HMGN1
Full Name
Non-histone chromosomal protein HMG-14
Weight
10.659kDa
Superfamily
HMGN family
Alternative Names
FLJ31471; High mobility group nucleosome-binding domain-containing protein 1; high-mobility group (nonhistone chromosomal) protein 14; high-mobility group nucleosome binding 1; high-mobility group nucleosome binding domain 1; HMG14; HMG14FLJ27265; HMGN1; MGC104230; MGC117425; non-histone chromosomal protein HMG-14; nonhistone chromosomal protein HMG-14 HMGN1 HMG14 high mobility group nucleosome binding domain 1 non-histone chromosomal protein HMG-14|high mobility group nucleosome-binding domain-containing protein 1|high-mobility group (nonhistone chromosomal) protein 14|high-mobility group nucleosome binding 1|nonhistone chromosomal protein HMG-14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMGN1, check out the HMGN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMGN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-HMG-14 (S21) HMGN1 Antibody (A03051S21)
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No publications found for A03051S21
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