Anti-Phospho IREB1 (Ser711) ACO1 Antibody

Aconitase 1 antibody

Boster Bio Anti-Phospho IREB1 (Ser711) ACO1 Antibody (Catalog# P30450). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Product Info Summary

SKU: P30450
Size: 100ul
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB, IF (paraffin)

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Product Name

Anti-Phospho IREB1 (Ser711) ACO1 Antibody

View all Aconitase 1 Antibodies

SKU/Catalog Number

P30450

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho IREB1 (Ser711) ACO1 Antibody (Catalog# P30450). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho IREB1 (Ser711) ACO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30450)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human IREB1 around the phosphorylation site of Ser711. Immunogen sequence location: 681-730

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P30450 is reactive to ACO1 in Human, Monkey, Mouse, Rat

Applications

P30450 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

98.399kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For ACO1 (Source: Uniprot.org, NCBI)

Gene Name

ACO1

Full Name

Cytoplasmic aconitate hydratase

Weight

98.399kDa

Superfamily

aconitase/IPM isomerase family

Alternative Names

aconitase 1, soluble; Aconitase; aconitate hydratase; Citrate hydro-lyase; cytoplasmic aconitate hydratase; EC 4.2.1; EC 4.2.1.3; Ferritin repressor protein; IREB1IREBP1; IRE-BP 1; IREBP; Iron regulatory protein 1; iron-responsive element binding protein 1; Iron-responsive element-binding protein 1; IRP1ACONS ACO1 ACONS, HEL60, IREB1, IREBP, IREBP1, IRP1 aconitase 1 cytoplasmic aconitate hydratase|aconitase 1, soluble|aconitate hydratase, cytoplasmic|citrate hydro-lyase|cytoplasmic aconitase|cytosplasmic aconitase|epididymis luminal protein 60|epididymis secretory sperm binding protein|ferritin repressor protein|iron regulatory protein 1|iron-responsive element-binding protein 1|soluble aconitase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACO1, check out the ACO1 Infographic

ACO1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Phospho IREB1 (Ser711) ACO1 Antibody

Question

Is there a BSA free version of anti-Phospho IREB1 (Ser711) antibody P30450 available?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho IREB1 (Ser711) antibody P30450 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-27

Question

Is this P30450 anti-Phospho IREB1 (Ser711) antibody reactive to the isotypes of ACO1?

Verified Customer

Verified customer

Asked: 2018-09-24

Answer

The immunogen of P30450 anti-Phospho IREB1 (Ser711) antibody is A peptide derived from human IREB1 around the phosphorylation site of Ser711. Immunogen sequence location: 681-730. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-24

Question

We are currently using anti-Phospho IREB1 (Ser711) antibody P30450 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2018-08-13

Answer

The anti-Phospho IREB1 (Ser711) antibody (P30450) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-13

Question

I see that the anti-Phospho IREB1 (Ser711) antibody P30450 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-08-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-08-03

Question

We want to test anti-Phospho IREB1 (Ser711) antibody P30450 on human liver for research purposes, then I may be interested in using anti-Phospho IREB1 (Ser711) antibody P30450 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-09-14

Answer

The products we sell, including anti-Phospho IREB1 (Ser711) antibody P30450, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-14

Question

My question regarding product P30450, anti-Phospho IREB1 (Ser711) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Jha

Verified customer

Asked: 2015-04-02

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P30450 anti-Phospho IREB1 (Ser711) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-04-02

Question

Please see the WB image, lot number and protocol we used for liver using anti-Phospho IREB1 (Ser711) antibody P30450. Please let me know if you require anything else.

D. Rodriguez

Verified customer

Asked: 2013-10-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-10-09

Size

Total: $335

SKU:P30450

Backordered.

Lead time for this item is typically 5-7 days

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In stock
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P30450
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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