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Product Info Summary
| SKU: | P30450 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho IREB1 (Ser711) ACO1 Antibody
SKU/Catalog Number
P30450
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho IREB1 (Ser711) ACO1 Antibody (Catalog# P30450). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho IREB1 (Ser711) ACO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30450)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human IREB1 around the phosphorylation site of Ser711. Immunogen sequence location: 681-730
Reactive Species
P30450 is reactive to ACO1 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
98.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30450 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using Phospho-IRP-1 (S711) Polyclonal Antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IREB1 (Phospho-Ser711) Antibody
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Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using IREB1 (Phospho-Ser711) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells treated with insulin 0.01U/ml 30', using IREB1 (Phospho-Ser711) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho IREB1 (Ser711) ACO1 Antibody (P30450)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Phospho IREB1 (Ser711) ACO1 Antibody
Question
Is there a BSA free version of anti-Phospho IREB1 (Ser711) antibody P30450 available?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho IREB1 (Ser711) antibody P30450 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-27
Question
Is this P30450 anti-Phospho IREB1 (Ser711) antibody reactive to the isotypes of ACO1?
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
The immunogen of P30450 anti-Phospho IREB1 (Ser711) antibody is A peptide derived from human IREB1 around the phosphorylation site of Ser711. Immunogen sequence location: 681-730. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-24
Question
We are currently using anti-Phospho IREB1 (Ser711) antibody P30450 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
The anti-Phospho IREB1 (Ser711) antibody (P30450) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-13
Question
I see that the anti-Phospho IREB1 (Ser711) antibody P30450 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-08-03
Question
We want to test anti-Phospho IREB1 (Ser711) antibody P30450 on human liver for research purposes, then I may be interested in using anti-Phospho IREB1 (Ser711) antibody P30450 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
The products we sell, including anti-Phospho IREB1 (Ser711) antibody P30450, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-14
Question
My question regarding product P30450, anti-Phospho IREB1 (Ser711) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Jha
Verified customer
Asked: 2015-04-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P30450 anti-Phospho IREB1 (Ser711) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-04-02
Question
Please see the WB image, lot number and protocol we used for liver using anti-Phospho IREB1 (Ser711) antibody P30450. Please let me know if you require anything else.
D. Rodriguez
Verified customer
Asked: 2013-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-09
