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Product Info Summary
| SKU: | P00165 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00165
BM4844 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody catalog # P00165. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00165)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IGO-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-IRF3 (S386)
Reactive Species
P00165 is reactive to IRF3 in Human
Observed Molecular Weight
55 kDa
Calculated molecular weight
47.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00165 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: human SIHA whole cell, human RT4 whole cell
Validation Images & Assay Conditions
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Western blot analysis of P-IRF3 using anti-P-IRF3 antibody (P00165).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-IRF3 antigen affinity purified monoclonal antibody (P00165) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-IRF3 at approximately 55 kDa. The expected band size for P-IRF3 is at 47 kDa.
Specific Publications For Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody (P00165)
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4 Customer Q&As for Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody
Question
We have observed staining in human retina. What should we do? Is anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody supposed to stain retina positively?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
According to literature retina does express IRF3. According to Uniprot.org, IRF3 is expressed in small intestine peyer's patch, retina, spleen, eye kidney, erythroleukemia, liver, among other tissues. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody P00165 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
S. Gonzalez
Verified customer
Asked: 2018-02-13
Answer
The anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody (P00165) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-13
Question
Our lab were satisfied with the WB result of your anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody. However we have seen positive staining in spleen cytoplasm using this antibody. Is that expected? Could you tell me where is IRF3 supposed to be expressed?
K. Huang
Verified customer
Asked: 2014-11-14
Answer
From literature, spleen does express IRF3. Generally IRF3 expresses in cytoplasm. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-11-14
Question
We ordered your anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody for IF on small intestine peyer's patch in the past. I am using human, and We intend to use the antibody for WB next. Our lab want to know about examining small intestine peyer's patch as well as spleen in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
D. Thomas
Verified customer
Asked: 2014-07-24
Answer
I viewed the website and datasheets of our anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody and it appears that P00165 has been validated on human in both IF and WB. Thus P00165 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-07-24
