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- Table of Contents
IRF3 antibody (P00165) performs well in IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Western blot analysis of P-IRF3 using anti-P-IRF3 antibody (P00165).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-IRF3 antigen affinity purified monoclonal antibody (P00165) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-IRF3 at approximately 55 kDa. The expected band size for P-IRF3 is at 47 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-IRF3 antigen affinity purified monoclonal antibody (P00165) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-IRF3 at approximately 55 kDa. The expected band size for P-IRF3 is at 47 kDa.
Designing a Western blot for IRF3? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the IRF3 Western Blot GuideBundle Save
Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody
IRF3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IF, ICC, WB |
Tested Applications
IRF3 antibody was positively detected by WB in:
human SIHA whole cell, human RT4 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB
1:500-2000
ICC/IF
1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody
View all IRF3 Antibodies
View all IRF3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IGO-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-IRF3 (S386)
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
47.2 kDa
Cite This Product
Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00165)
Protocols
Western Blot protocol for IRF3 antibody
Publications
Application:
Species:
Electroacupuncture protects gastric Cajal cells by reducing macrophage pyroptosis in diabetic gastroparesis
Published: 2026-02-15
Journal: World Journal of Diabetes
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 41696103
JMJD2A promotes the development of castration-resistant prostate cancer by activating androgen receptor enhancer and inhibiting the cGAS-STING pathway
Published: 2024-05-31
Journal: MOLECULAR CARCINOGENESIS
Application: IF,WB
Impact Factor: 3.00
Species: Human,Mouse
PMID: 38818897
Review
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1 Review For Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody
Anti-pIRF3 Antibody (BM4844) produced sharp target bands in WB assays on mouse macrophages with faint non-specific bands, and β-tubulin served as the loading control.
| SKU | P00165 |
|---|---|
| Application | Western Blot |
| Sample | Mouse macrophages |
| Sample Processing Description | Cells were lysed in RIPA lysis buffer supplemented with PMSF (100:1) for 10 min. The lysates were centrifuged at 12,000 rpm for 15 min, and the supernatants were collected and mixed with 5× Loading Buffer. Samples were denatured at 100°C for 10 min and then loaded onto an SDS-PAGE gel. |
| Other Reagents | Blocking buffer: 5% non-fat milk |
| Primary Antibody | Phospho-IRF3 (S386) Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2,000 dilution, incubated overnight at 4°C. |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG secondary antibody |
| Secondary Incubation | 1:2000, incubated for 1 hour at RT.< |
| Detection | ECL, Imaging system: UNITEC CAMBRIDGE |
| Results Summary | The figure displays Western blot results for the target protein pIRF3 and the internal control β-tubulin in various types of macrophages. The target bands are clear and distinct, and the experimental results are satisfactory. |
Jingting Jia, Capital Medical University
Verified customer
Submitted 2026-11-05
Product Promise
We promise Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00165
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 27, 2026