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Product Info Summary
| SKU: | P30451 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody
SKU/Catalog Number
P30451
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody (Catalog# P30451). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30451)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MKP-1/2 around the phosphorylation site of Ser296/318. Immunogen sequence location: 261-310
Reactive Species
P30451 is reactive to DUSP1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
39.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30451 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:5000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of JK cells using Phospho-MKP-1/2 (S296/318) Polyclonal Antibody.
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Western Blot analysis of various cells using Phospho-MKP-1/2 (S296/318) Polyclonal Antibody.
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Western blot analysis of lysates from K562 cells treated with heat shock , using MKP-1/2 (Phospho-Ser296/318) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody (P30451)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody
Question
Is a blocking peptide available for product anti-Phospho MKP-1/2 (Ser296/318) antibody (P30451)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do provide the blocking peptide for product anti-Phospho MKP-1/2 (Ser296/318) antibody (P30451). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-23
Question
We are currently using anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Z. Thomas
Verified customer
Asked: 2019-12-16
Answer
The anti-Phospho MKP-1/2 (Ser296/318) antibody (P30451) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
Will anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 work on goat IHC with foreskin?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
Our lab technicians have not validated anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat foreskin in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-02
Question
We need to test anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 on mouse brain for research purposes, then I may be interested in using anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The products we sell, including anti-Phospho MKP-1/2 (Ser296/318) antibody P30451, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-14
Question
Here is the WB image, lot number and protocol we used for brain using anti-Phospho MKP-1/2 (Ser296/318) antibody P30451. Please let me know if you require anything else.
N. Krishna
Verified customer
Asked: 2015-10-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-13
