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Product Info Summary
| SKU: | P04660 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho NEK9 (Thr210) Antibody
SKU/Catalog Number
P04660
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho NEK9 (Thr210) Antibody (Catalog# P04660). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho NEK9 (Thr210) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04660)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human NEK9 around the phosphorylation site of Thr210. Immunogen sequence location: 176-225
Reactive Species
P04660 is reactive to NEK9 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
107.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P04660 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NEK9 (Phospho-Thr210) Antibody
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Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HepG2 cells, using NEK9 (Phospho-Thr210) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho NEK9 (Thr210) Antibody (P04660)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho NEK9 (Thr210) Antibody
Question
I was wanting to use your anti-Phospho NEK9 (Thr210) antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
It shows on the product datasheet, P04660 anti-Phospho NEK9 (Thr210) antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-27
Question
We want to test anti-Phospho NEK9 (Thr210) antibody P04660 on rat leukemic t-cell for research purposes, then I may be interested in using anti-Phospho NEK9 (Thr210) antibody P04660 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Walker
Verified customer
Asked: 2019-10-25
Answer
The products we sell, including anti-Phospho NEK9 (Thr210) antibody P04660, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-25
Question
We are currently using anti-Phospho NEK9 (Thr210) antibody P04660 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The anti-Phospho NEK9 (Thr210) antibody (P04660) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-18
Question
Does anti-Phospho NEK9 (Thr210) antibody P04660 work for WB with leukemic t-cell?
L. Krishna
Verified customer
Asked: 2018-05-08
Answer
According to the expression profile of leukemic t-cell, NEK9 is highly expressed in leukemic t-cell. So, it is likely that anti-Phospho NEK9 (Thr210) antibody P04660 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2018-05-08
