Product Info Summary
SKU: | A06026S357 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Net (S357) ELK3 Antibody
SKU/Catalog Number
A06026S357
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Net (S357) ELK3 Antibody catalog # A06026S357. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Net (S357) ELK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06026S357)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Net around the phosphorylation site of S357.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06026S357 is reactive to ELK3 in Human, Monkey, Mouse
Applications
A06026S357 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44.24kDa
Background of ELK3
The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity.
Dewaste V., Biochem. Biophys. Res. Commun. 291:400-405(2002).
Bertsch U., Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
Gregory S.G., Nature 441:315-321(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of ELK3 using Anti-Phospho-Net (S357) ELK3 Antibody (A06026S357).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°CClick image to see more details
Figure 1. Western blotting validation for Anti-Phospho-Net (S357) ELK3 Antibody A06026S357
Western Blot (WB) analysis of COLO205 cells using Phospho-Net (S357) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ELK3 (Source: Uniprot.org, NCBI)
Gene Name
ELK3
Full Name
ETS domain-containing protein Elk-3
Weight
44.24kDa
Superfamily
ETS family
Alternative Names
ELK3, ETS-domain protein (SRF accessory protein 2); ERPETS domain-containing protein Elk-3; ETS-domain protein; ETS-related protein ERP; NETSRF accessory protein 2; SAP-2; SAP2ETS-related protein NET; Serum response factor accessory protein 2 ELK3 ERP, NET, SAP-2, SAP2 ETS transcription factor ELK3 ETS domain-containing protein Elk-3|ELK3, ETS transcription factor|ELK3, ETS-domain protein (SRF accessory protein 2)|ETS-related protein ERP|ETS-related protein NET|SRF accessory protein 2|serum response factor accessory protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ELK3, check out the ELK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Net (S357) ELK3 Antibody (A06026S357)
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No publications found for A06026S357
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Customer Reviews
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