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Product Info Summary
| SKU: | P01815 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho NFAT5 (Ser1197) Antibody
SKU/Catalog Number
P01815
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho NFAT5 (Ser1197) Antibody (Catalog# P01815). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho NFAT5 (Ser1197) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01815)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human NFAT5 around the phosphorylation site of Ser1197. Immunogen sequence location: 1171-1220
Reactive Species
P01815 is reactive to NFAT5 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
165.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01815 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NFAT5 (Phospho-Ser1197) Antibody
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Immunofluorescence analysis of COS7 cells, using NFAT5 (Phospho-Ser1197) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho NFAT5 (Ser1197) Antibody (P01815)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Phospho NFAT5 (Ser1197) Antibody
Question
I would like to test anti-Phospho NFAT5 (Ser1197) antibody P01815 on human kidney for research purposes, then I may be interested in using anti-Phospho NFAT5 (Ser1197) antibody P01815 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The products we sell, including anti-Phospho NFAT5 (Ser1197) antibody P01815, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-06
Question
We are currently using anti-Phospho NFAT5 (Ser1197) antibody P01815 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
The anti-Phospho NFAT5 (Ser1197) antibody (P01815) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-03
Question
Is there a BSA free version of anti-Phospho NFAT5 (Ser1197) antibody P01815 available?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho NFAT5 (Ser1197) antibody P01815 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-27
Question
Does anti-Phospho NFAT5 (Ser1197) antibody P01815 work on horse IF with kidney?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
Our lab technicians have not tested anti-Phospho NFAT5 (Ser1197) antibody P01815 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Phospho NFAT5 (Ser1197) antibody P01815 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse kidney in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-18
Question
I see that the anti-Phospho NFAT5 (Ser1197) antibody P01815 works with IF, what is the protocol used to produce the result images on the product page?
K. Singh
Verified customer
Asked: 2017-10-09
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-10-09
Question
I was wanting to use your anti-Phospho NFAT5 (Ser1197) antibody for IF for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?
P. Thomas
Verified customer
Asked: 2016-03-30
Answer
As indicated on the product datasheet, P01815 anti-Phospho NFAT5 (Ser1197) antibody has been tested for IF on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-30
