Product Info Summary
SKU: | M00497T497 |
---|---|
Size: | 100 µl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-PKC alpha (T497) Rabbit Monoclonal Antibody
SKU/Catalog Number
M00497T497
Size
100 µl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-PKC alpha (T497) Rabbit Monoclonal Antibody catalog # M00497T497. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PKC alpha (T497) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00497T497)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
32P00
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-PKC alpha (T497)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00497T497 is reactive to PRKCA in Human, Mouse, Rat
Applications
M00497T497 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
76.75kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Phospho-PKC alpha (T497) expression in HeLa treated with Calyculin A cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For PRKCA (Source: Uniprot.org, NCBI)
Gene Name
PRKCA
Full Name
Protein kinase C alpha type
Weight
76.75kDa
Superfamily
protein kinase superfamily
Alternative Names
AAG6; aging-associated gene 6; EC 2.7.11; EC 2.7.11.13; MGC129901; PKC alpha; PKCA; PKC-A; PKC-alpha; PKCAMGC129900; PRKACA; PRKCA; protein kinase C alpha type; protein kinase C, alpha PRKCA AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA protein kinase C alpha protein kinase C alpha type|PKC-A|aging-associated gene 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCA, check out the PRKCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PKC alpha (T497) Rabbit Monoclonal Antibody (M00497T497)
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