Overview
| Product Name |
Anti-Phospho POLR2A (Ser1619) Antibody
See more RNA Polymerase II/POLR2A products |
| Catalog# |
P30431 |
| Pack Size |
100ul |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-Phospho POLR2A (Ser1619) Antibody catalog # P30431. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. |
| Cite This Product |
Anti-Phospho POLR2A (Ser1619) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30431)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
IF, IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A peptide derived from human POLR2A around the phosphorylation site of Ser1619. Immunogen sequence location: 1585-1634 |
Gene/Protein Basic Information For POLR2A (Source: Uniprot.org, NCBI)
| Uniprot Id | P24928 |
|---|
| NCBI Gene Id | 5430 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | POLR2A |
|---|
| Protein Name | DNA-directed RNA polymerase II subunit RPB1 |
|---|
| Superfamily | RNA polymerase beta' chain family |
|---|
| Alternative Names | RNA Polymerase II/POLR2A|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.48; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; POLR2; POLR2A; POLRA; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; RNA Pol 2; RNA Polymerase 2; RNA polymerase II subunit B1; RNA Polymerase II; RNA-directed RNA polymerase II subunit RPB1; RNAPII; RPB1; RPBh1; RpIILS; RPO2; RPOL2 |
|---|
| Molecular Weight | 217176 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on POLR2A, check out the POLR2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for POLR2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the POLR2A infographic
Our Boster Quality Guarantee for Anti-Phospho POLR2A (Ser1619) Antibody covers its use in the following applications.
User need to optimize the dilution ratio for this antibody
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
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Total number of citations: 1
| Heparanase mediates vascular endothelial growth factor gene transcription in high-glucose human retinal microvascular endothelial cells. |
PubMed ID 28848320 |
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Questions and answers from customers related to P30431 Anti-Phospho POLR2A (Ser1619) Antibody
3 Related Questions
Question
My lab would like to test anti-Phospho POLR2A (Ser1619) antibody P30431 on human brain testis for research purposes, then I may be interested in using anti-Phospho POLR2A (Ser1619) antibody P30431 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Asked: 2019-05-28
Answer
The products we sell, including anti-Phospho POLR2A (Ser1619) antibody P30431, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-28
Question
Is there a BSA free version of anti-Phospho POLR2A (Ser1619) antibody P30431 available?
Verified Customer
Asked: 2017-09-07
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho POLR2A (Ser1619) antibody P30431 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-07
Question
Would anti-Phospho POLR2A (Ser1619) antibody P30431 work for IHC with brain testis?
Answer
According to the expression profile of brain testis, POLR2A is highly expressed in brain testis. So, it is likely that anti-Phospho POLR2A (Ser1619) antibody P30431 will work for IHC with brain testis.
Boster Scientific Support
Answered: 2015-01-23
