Anti-Phospho POLR2A (Ser1619) Antibody

Product Name

Anti-Phospho POLR2A (Ser1619) Antibody

See all RNA Polymerase II/POLR2A products

SKU/Catalog Number

P30431

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho POLR2A (Ser1619) Antibody catalog # P30431. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho POLR2A (Ser1619) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30431)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

A peptide derived from human POLR2A around the phosphorylation site of Ser1619. Immunogen sequence location: 1585-1634

Reactive Species

P30431 is reactive to POLR2A in Human, Mouse, Rat

Applications

P30431 is guaranteed for IF, IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.

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Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.

Gene/Protein Information For POLR2A (Source: Uniprot.Org, NCBI)

Alternative Names

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.48; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; POLR2; POLR2A; POLRA; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; RNA Pol 2; RNA Polymerase 2; RNA polymerase II subunit B1; RNA Polymerase II; RNA-directed RNA polymerase II subunit RPB1; RNAPII; RPB1; RPBh1; RpIILS; RPO2; RPOL2 POLR2A NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1|polymerase (RNA) II (DNA directed) polypeptide A, 220kDa|polymerase (RNA) II subunit A

For more info on POLR2A, check out the POLR2A Infographic

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In this infographic you will see the following information for POLR2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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