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Product Info Summary
| SKU: | P30431 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho POLR2A (Ser1619) Antibody
SKU/Catalog Number
P30431
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho POLR2A (Ser1619) Antibody (Catalog# P30431). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho POLR2A (Ser1619) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30431)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human POLR2A around the phosphorylation site of Ser1619. Immunogen sequence location: 1585-1634
Reactive Species
P30431 is reactive to POLR2A in Human, Monkey, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
217.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30431 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of VEC using p-Rpb1 (S1619) antibody. Antibody was diluted at 1:2000.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using POLR2A (Phospho-Ser1619) Antibody
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Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho POLR2A (Ser1619) Antibody (P30431)
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Customer Q&As
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3 Customer Q&As for Anti-Phospho POLR2A (Ser1619) Antibody
Question
My lab would like to test anti-Phospho POLR2A (Ser1619) antibody P30431 on human brain testis for research purposes, then I may be interested in using anti-Phospho POLR2A (Ser1619) antibody P30431 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The products we sell, including anti-Phospho POLR2A (Ser1619) antibody P30431, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-28
Question
Is there a BSA free version of anti-Phospho POLR2A (Ser1619) antibody P30431 available?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho POLR2A (Ser1619) antibody P30431 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-07
Question
Would anti-Phospho POLR2A (Ser1619) antibody P30431 work for IHC with brain testis?
J. Li
Verified customer
Asked: 2015-01-23
Answer
According to the expression profile of brain testis, POLR2A is highly expressed in brain testis. So, it is likely that anti-Phospho POLR2A (Ser1619) antibody P30431 will work for IHC with brain testis.
Boster Scientific Support
Answered: 2015-01-23
