Product Info Summary
SKU: | A32113Y413 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-Pragmin (Y413) PRAG1 Antibody
SKU/Catalog Number
A32113Y413
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Pragmin (Y413) PRAG1 Antibody catalog # A32113Y413. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Pragmin (Y413) PRAG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32113Y413)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Pragmin around the phosphorylation site of Y413.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A32113Y413 is reactive to PRAG1 in Human, Mouse, Rat
Applications
A32113Y413 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
149.624kDa
Background of PRAG1
Catalytic subunit of a TRAMP-like complex which has a poly (A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo (A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Phospho-Pragmin (Y413) PRAG1 Antibody A32113Y413
Western Blot (WB) analysis of A549 cells using Phospho-Pragmin (Y413) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PRAG1 (Source: Uniprot.org, NCBI)
Gene Name
PRAG1
Full Name
Inactive tyrosine-protein kinase PRAG1
Weight
149.624kDa
Superfamily
protein kinase superfamily
Alternative Names
Inactive tyrosine-protein kinase PRAG1 PRAG1 NACK, PEAK2, PRAGMIN, SGK223 PEAK1 related, kinase-activating pseudokinase 1 inactive tyrosine-protein kinase PRAG1|PEAK family member 2|Pragmin, RND2 effector protein|homolog of rat pragma of Rnd2|sugen kinase 223|tyrosine-protein kinase PRAG1|tyrosine-protein kinase SgK223
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRAG1, check out the PRAG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Pragmin (Y413) PRAG1 Antibody (A32113Y413)
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No publications found for A32113Y413
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