Click image to see more details
Product Info Summary
| SKU: | M00039-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-Retinoblastoma (S780) RB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00039-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Retinoblastoma (S780) RB1 Rabbit Monoclonal Antibody catalog # M00039-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Retinoblastoma (S780) RB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00039-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BHC-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Retinoblastoma
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00039-1 is reactive to RB1 in Human
Applications
M00039-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
83, 88 kDa
Calculated molecular weight
106.159kDa
Background of RB1
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of Retinoblastoma phosphorylation expression in Jurkat cell lysate treated with Alkaline Phosphatase.
Protein Target Info & Infographic
Gene/Protein Information For RB1 (Source: Uniprot.org, NCBI)
Gene Name
RB1
Full Name
Retinoblastoma-associated protein
Weight
106.159kDa
Superfamily
retinoblastoma protein (RB) family
Alternative Names
OSRC; osteosarcoma; p105-Rb; pp110; pRb; RB; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; retinoblastoma-associated protein RB1 OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110 RB transcriptional corepressor 1 retinoblastoma-associated protein|GOS563 exon 17 substitution mutation causes premature stop|exon 17 tumor GOS561 substitution mutation causes premature stop|prepro-retinoblastoma-associated protein|protein phosphatase 1, regulatory subunit 130|retinoblastoma 1|retinoblastoma suspectibility protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RB1, check out the RB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Retinoblastoma (S780) RB1 Rabbit Monoclonal Antibody (M00039-1)
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No publications found for M00039-1
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Phospho-Retinoblastoma (S780) RB1 Rabbit Monoclonal Antibody
Question
Would anti-Phospho-Retinoblastoma (S780) Rabbit Monoclonal antibody M00039-1 work for IHC with endometrium?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
According to the expression profile of endometrium, RB1 is highly expressed in endometrium. So, it is likely that anti-Phospho-Retinoblastoma (S780) Rabbit Monoclonal antibody M00039-1 will work for IHC with endometrium.
Boster Scientific Support
Answered: 2019-11-29
Question
I have a question about product M00039-1, anti-Phospho-Retinoblastoma (S780) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Kulkarni
Verified customer
Asked: 2016-12-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00039-1 anti-Phospho-Retinoblastoma (S780) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-12-01
Question
Please see the WB image, lot number and protocol we used for endometrium using anti-Phospho-Retinoblastoma (S780) Rabbit Monoclonal antibody M00039-1. Please let me know if you require anything else.
D. Gonzalez
Verified customer
Asked: 2015-08-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-08-11
