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Product Info Summary
| SKU: | P00018 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00018
BM4689 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody catalog # P00018. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00018)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HAI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SIRT1
Reactive Species
P00018 is reactive to SIRT1 in Human
Observed Molecular Weight
120 kDa
Calculated molecular weight
81.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00018 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: 293T cell, 293T cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-SIRT1 (T530) expression in (1) 293T cell lysate; (2) 293T cell treated with Calyculin A.
Specific Publications For Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody (P00018)
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Customer Reviews
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4 Customer Q&As for Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody P00018 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
J. Zhao
Verified customer
Asked: 2020-04-07
Answer
The anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody (P00018) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
We are interested in using your anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody for protein deacetylation studies. Has this antibody been tested with western blotting on 293t cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
We appreciate your inquiry. This P00018 anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody is validated on 293t cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-08
Question
We have observed staining in human cervix carcinoma. What should we do? Is anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to literature cervix carcinoma does express SIRT1. According to Uniprot.org, SIRT1 is expressed in right adrenal gland, testis, prostate, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2019-08-12
Question
We were happy with the WB result of your anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody. However we have observed positive staining in leukemic t-cell sirtt1 75 kda fragment: cytoplasm using this antibody. Is that expected? Could you tell me where is SIRT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
Based on literature, leukemic t-cell does express SIRT1. Generally SIRT1 expresses in nucleus, pml body, sirtt1 75 kda fragment: cytoplasm. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2019-06-28
