Product Info Summary
SKU: | P02148 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-SMC1 (S966) SMC1A Antibody
SKU/Catalog Number
P02148
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-SMC1 (S966) SMC1A Antibody catalog # P02148. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-SMC1 (S966) SMC1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02148)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
608
Isotype
IgG
Immunogen
Synthesized peptide derived from human SMC1 around the phosphorylation site of S966.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P02148 is reactive to SMC1A in Human, Mouse, Rat
Applications
P02148 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
143.233kDa
Background of SMC1
REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr-25, Thr-23 and Ser-19 through the activity of GSK3b (Katiyar et al, 2009).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of K562 using p-SMC1 (S966) antibody.
Protein Target Info & Infographic
Gene/Protein Information For SMC1A (Source: Uniprot.org, NCBI)
Gene Name
SMC1A
Full Name
Structural maintenance of chromosomes protein 1A
Weight
143.233kDa
Superfamily
SMC family
Alternative Names
CDLS2; DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast); KIAA0178DKFZp686L19178; SB1.8; SB1.8MGC138332; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1; SMC1A; SMC-1A; SMC-1-alpha; SMC1L1; SMC1SMC1alpha; Smcb; structural maintenance of chromosomes 1A; structural maintenance of chromosomes protein 1A SMC1A CDLS2, DEE85, DXS423E, EIEE85, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB structural maintenance of chromosomes 1A structural maintenance of chromosomes protein 1A|SMC protein 1A|SMC-1-alpha|SMC1 (structural maintenance of chromosomes 1, yeast)-like 1|epididymis secretory sperm binding protein|segregation of mitotic chromosomes 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMC1A, check out the SMC1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMC1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-SMC1 (S966) SMC1A Antibody (P02148)
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No publications found for P02148
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-SMC1 (S966) SMC1A Antibody
Question
We are currently using anti-Phospho-SMC1 (S966) antibody P02148 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The anti-Phospho-SMC1 (S966) antibody (P02148) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-01