Click image to see more details
Product Info Summary
| SKU: | MP00107 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Phospho-Src (Y419) Monoclonal Antibody
SKU/Catalog Number
MP00107
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Src (Y419) Monoclonal Antibody catalog # MP00107. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Src (Y419) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00107)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DDH-19
Immunogen
A synthesized peptide derived from human Phospho-Src (Y419)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
MP00107 is reactive to SRC in Human, Mouse, Rat
Applications
MP00107 is guaranteed for Flow Cytometry, WB Boster Guarantee
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000
FC 1:30
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SRC using anti-SRC antibody (MP00107) in A431 cell lysate treated with pervanadate.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRC antigen affinity purified polyclonal antibody (Catalog # MP00107) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Protein Target Info & Infographic
Gene/Protein Information For SRC (Source: Uniprot.Org, NCBI)
Uniprot ID
P12931
Gene ID
6714
Gene Name
SRC
Full Name
Proto-oncogene tyrosine-protein kinase Src
Weight
59.835kDa
Superfamily
protein kinase superfamily
Alternative Names
ASV; c-Src; EC 2.7.10; EC 2.7.10.2; pp60c-src; Rous sarcoma; RSVgp4; Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) SRC ASV1, THC6, c-SRC, p60-Src, SRC SRC proto-oncogene, non-receptor tyrosine kinase proto-oncogene tyrosine-protein kinase Src|proto-oncogene c-Src|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1|v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SRC, check out the SRC Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SRC: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-Phospho-Src (Y419) Monoclonal Antibody (MP00107)
MP00107 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang J,Cui J,Li X,Hao X,Guo L,Wang H,Liu H.Increased secretion of VEGF-C from SiO2-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis.Ecotoxicol Environ Saf.2021 Apr 29;218:112257.doi:10.1016/j.ecoenv.2021.112257.Epub ahead of print.PMID:33933809.
Species: Human
MP00107 usage in article: APP:WB, SAMPLE:LECS, DILUTION:NA
Zhang Qq, Zhou Dl, Lei Y, Zheng L, Chen Sx, Gou Hj, Gu Ql, He Xd, Lan T, Qi Cl, Li Jc, Ding Yq, Qiao L, Wang Lj. Oncotarget. 2015 Feb 20;6(5):3123-35. Slit2/Robo1 Signaling Promotes Intestinal Tumorigenesis Through Src-Mediated Activation Of The W...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Phospho-Src (Y419) Monoclonal Antibody
Question
I was wanting to use your anti-Phospho-Src (Y419) Monoclonal antibody for Flow Cytometry for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
You can see on the product datasheet, MP00107 anti-Phospho-Src (Y419) Monoclonal antibody has been validated for Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-20
Question
We are currently using anti-Phospho-Src (Y419) Monoclonal antibody MP00107 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The anti-Phospho-Src (Y419) Monoclonal antibody (MP00107) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-27
Question
My team were well pleased with the WB result of your anti-Phospho-Src (Y419) Monoclonal antibody. However we have observed positive staining in liver cell membrane. using this antibody. Is that expected? Could you tell me where is SRC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
Based on literature, liver does express SRC. Generally SRC expresses in cell membrane. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-02-21
Question
Is a blocking peptide available for product anti-Phospho-Src (Y419) Monoclonal antibody (MP00107)?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
We do provide the blocking peptide for product anti-Phospho-Src (Y419) Monoclonal antibody (MP00107). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-06
Question
I have a question about product MP00107, anti-Phospho-Src (Y419) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MP00107 anti-Phospho-Src (Y419) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-17
Question
We have tried in the past anti-Phospho-Src (Y419) Monoclonal antibody for Flow Cytometry on leukemic t-cell in the past. I am using human, and We intend to use the antibody for WB next. My lab would like examining leukemic t-cell as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
A. Roberts
Verified customer
Asked: 2018-09-04
Answer
I viewed the website and datasheets of our anti-Phospho-Src (Y419) Monoclonal antibody and it seems that MP00107 has been validated on human in both Flow Cytometry and WB. Thus MP00107 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-04
Question
Is this MP00107 anti-Phospho-Src (Y419) Monoclonal antibody reactive to the isotypes of SRC?
E. Bhatt
Verified customer
Asked: 2018-04-12
Answer
The immunogen of MP00107 anti-Phospho-Src (Y419) Monoclonal antibody is A synthesized peptide derived from human Phospho-Src (Y419). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-12
Question
Do you have a BSA free version of anti-Phospho-Src (Y419) Monoclonal antibody MP00107 available?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Src (Y419) Monoclonal antibody MP00107 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Phospho-Src (Y419) Monoclonal antibody MP00107. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-25
Question
My question regards to test anti-Phospho-Src (Y419) Monoclonal antibody MP00107 on human liver for research purposes, then I may be interested in using anti-Phospho-Src (Y419) Monoclonal antibody MP00107 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Miller
Verified customer
Asked: 2017-10-27
Answer
The products we sell, including anti-Phospho-Src (Y419) Monoclonal antibody MP00107, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-27
Question
Here is the WB image, lot number and protocol we used for liver using anti-Phospho-Src (Y419) Monoclonal antibody MP00107. Please let me know if you require anything else.
R. Mitchell
Verified customer
Asked: 2017-05-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-18
Question
I was wanting to use using your anti-Phospho-Src (Y419) Monoclonal antibody for intracellular signal transduction studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
A. Johnson
Verified customer
Asked: 2016-03-30
Answer
Thank you for your inquiry. This MP00107 anti-Phospho-Src (Y419) Monoclonal antibody is validated on a431 cell lysate. It is guaranteed to work for Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-03-30
Question
We have seen staining in human erythroleukemia. Any tips? Is anti-Phospho-Src (Y419) Monoclonal antibody supposed to stain erythroleukemia positively?
V. Krishna
Verified customer
Asked: 2015-10-26
Answer
Based on literature erythroleukemia does express SRC. Based on Uniprot.org, SRC is expressed in fundus of stomach, lung skin, platelet, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, among other tissues. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2015-10-26
Question
I see that the anti-Phospho-Src (Y419) Monoclonal antibody MP00107 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
W. Collins
Verified customer
Asked: 2014-08-22
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-08-22
Question
Does MP00107 anti-Phospho-Src (Y419) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Dhar
Verified customer
Asked: 2014-03-26
Answer
As indicated on the product datasheet, MP00107 anti-Phospho-Src (Y419) Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-03-26
Question
Will anti-Phospho-Src (Y419) Monoclonal antibody MP00107 work for Flow Cytometry with liver?
D. Huang
Verified customer
Asked: 2013-04-05
Answer
According to the expression profile of liver, SRC is highly expressed in liver. So, it is likely that anti-Phospho-Src (Y419) Monoclonal antibody MP00107 will work for Flow Cytometry with liver.
Boster Scientific Support
Answered: 2013-04-05
