Overview
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse, Rat |
| Applications |
Flow Cytometry, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A synthesized peptide derived from human Phospho-Src (Y419) |
Gene/Protein Basic Information For SRC (Source: Uniprot.org, NCBI)
| Uniprot Id | P12931 |
|---|
| NCBI Gene Id | 6714 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | SRC |
|---|
| Protein Name | Proto-oncogene tyrosine-protein kinase Src |
|---|
| Superfamily | protein kinase superfamily |
|---|
| Alternative Names | SRC|ASV; c-Src; EC 2.7.10; EC 2.7.10.2; pp60c-src; Rous sarcoma; RSVgp4; Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
|---|
| Molecular Weight | 59835 |
|---|
| Post Tranlational Modifications | Phosphorylation; Dephosphorylation; Cleavage; Myristoylation; Ubiquitination; Methylation; Palmitoylation; Reduction; Acetylation; Oxidation; Biotinylation; Acylation; Glycosylation; Ribosylation; Nitration; Prenylation; Nitrosylation; Farnesylation; Demethylation; Amidation |
|---|
| Subcellular Localization | Cell membrane; Lipid-anchor. Mitochondrion inner membrane. Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell junction, focal adhesion. Localizes to focal adhesion sites following integrin engagement (PubMed:22801373). Localization to focal adhesion sites requires myristoylation and the SH3 domain (PubMed:7525268). Colocalizes with PDLIM4 at the perinuclear region, but not at focal adhesions (PubMed:19307596). |
|---|
| Gene Location | 20q11.23, on Chromasome 20, gene sequence: NC_000020.11 (37344685..37406050) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For SRC (Source: Uniprot.org, NCBI)
| Protein Function | Non-receptor protein tyrosine kinase that is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein- coupled receptors as well as cytokine receptors. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be among the principal kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes in which it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an essential role in the regulation of cytoskeletal organization through phosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 permits the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled via the phosphorylation of cortactin (CTTN) (Probable). When cells stick via focal adhesions to the extracellular matrix, signals are transmitted by integrins to the cell leading to tyrosine phosphorylation of a range of focal adhesion proteins, including PTK2/FAK1 and paxillin (PXN) (PubMed:21411625). In addition to phosphorylating focal adhesion proteins, SRC is also active in the sites of cell-cell contact adherens junctions and phosphorylates substrates such as beta- catenin (CTNNB1), delta-catenin (CTNND1), and plakoglobin (JUP). Another type of cell-cell junction, the gap junction, is also a target for SRC, which phosphorylates connexin-43 (GJA1). SRC is implicated in regulation of pre-mRNA-processing and phosphorylates RNA-binding proteins such as KHDRBS1 (Probable). Also plays a role in PDGF-mediated tyrosine phosphorylation of both STAT1 and STAT3, leading to increased DNA binding activity of the transcription factors (By similarity). Involved in the RAS pathway through phosphorylation of RASA1 and RASGRF1 (PubMed:11389730). Plays a role in EGF-mediated calcium-activated chloride channel activation (PubMed:18586953). Required for epidermal growth factor receptor (EGFR) internalization via phosphorylation of clathrin heavy chain (CLTC and CLTCL1) in'Tyr-1477'. Involved in beta-arrestin (ARRB1 and ARRB2) desensitization via phosphorylation and activation of GRK2, leading to beta-arrestin phosphorylation and internalization. Has a crucial role in the stimulation of this CDK20/MAPK3 mitogen-activated protein kinase cascade by epidermal growth factor (Probable). Might be involved not only in mediating the transduction of mitogenic signals in the level of the plasma membrane but also in controlling progression through the cell cycle through interaction with regulatory proteins in the nucleus (PubMed:7853507). Plays an essential role in osteoclastic bone resorption in conjunction with PTK2B/PYK2. Both the formation of a SRC-PTK2B/PYK2 complicated and SRC kinase activity are essential for this function. Recruited to activated integrins by PTK2B/PYK2, thereby phosphorylating CBL, which then induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that's vital for osteoclast function (PubMed:8755529, PubMed:14585963). Promotes energy production in osteoclasts by triggering mitochondrial cytochrome C oxidase (PubMed:12615910). Phosphorylates DDR2 on tyrosine residues, thereby boosting its subsequent autophosphorylation (PubMed:16186108). Enhances DDX58/RIG-I-elicited antiviral signaling (PubMed:19419966). Phosphorylates PDPK1 in'Tyr-9','Tyr-373' and'Tyr-376' (PubMed:14585963). Phosphorylates BCAR1 in'Tyr-128' (PubMed:22710723). Phosphorylates CBLC at multiple tyrosine residues, phosphorylation in'Tyr-341' activates CBLC E3 action (PubMed:20525694). Involved in anchorage-independent cell growth (PubMed:19307596). |
|---|
| Tissue Specificity | Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. |
|---|
| Related Diseases | Malignant Neoplasms; Neoplasms; Tissue Adhesions; Cell Transformation, Neoplastic; Sarcoma; Neoplasm Metastasis; Carcinoma; Cell Invasion; Leukemia; Malignant Neoplasm Of Breast; Mammary Neoplasms; Tumor Angiogenesis; Malignant Paraganglionic Neoplasm; Inflammation; Neoplasm Invasiveness; Metaplastic Cell Transformation; Myeloid Leukemia; Adenocarcinoma |
|---|
| Related Pathways | Cell Proliferation; Localization; Cell Migration; Cell Growth; Cell Adhesion; Cell Cycle; Angiogenesis; Secretion; Endocytosis; Pathogenesis; Cell Activation; Cell Death; Cell Motility; Transport; T Cell Activation; Rna Interference; Translation; Immune Response; Chemotaxis; Cell Differentiation |
|---|
| Scientific References | PMID: 3299057 by Tanaka A., et al. DNA sequence encoding the amino-terminal region of the human c-src protein: implications of sequence divergence among src-type kinase oncogenes.
PMID: 2582238 by Anderson S.K., et al. Human cellular src gene: nucleotide sequence and derived amino acid sequence of the region coding for the carboxy-terminal two-thirds of pp60c-src. |
|---|
Our Boster Quality Guarantee for Anti-Phospho-Src (Y419) Monoclonal Antibody covers its use in the following applications.
WB 1:500~1:2000
FC 1:30
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Western blot analysis of SRC using anti-SRC antibody (MP00107) in A431 cell lysate treated with pervanadate.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRC antigen affinity purified polyclonal antibody (Catalog # MP00107) at 0.5 ug/mL overnight at 4
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
| Zhang Qq, Zhou Dl, Lei Y, Zheng L, Chen Sx, Gou Hj, Gu Ql, He Xd, Lan T, Qi Cl, Li Jc, Ding Yq, Qiao L, Wang Lj. Oncotarget. 2015 Feb 20;6(5):3123-35. Slit2/Robo1 Signaling Promotes Intestinal Tumorigenesis Through Src-Mediated Activation Of The W... |
PubMed ID 25605242 |
Submit your review to [email protected] or to Biocompare.com for an Amazon giftcard.
0 reviews
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to MP00107 Anti-Phospho-Src (Y419) Monoclonal Antibody
16 Related Questions
Question
I was wanting to use your anti-Phospho-Src (Y419) Monoclonal antibody for Flow Cytometry for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Asked: 2020-03-20
Answer
You can see on the product datasheet, MP00107 anti-Phospho-Src (Y419) Monoclonal antibody has been validated for Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-20
Question
We are currently using anti-Phospho-Src (Y419) Monoclonal antibody MP00107 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Asked: 2019-11-27
Answer
The anti-Phospho-Src (Y419) Monoclonal antibody (MP00107) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-27
Question
My team were well pleased with the WB result of your anti-Phospho-Src (Y419) Monoclonal antibody. However we have observed positive staining in liver cell membrane. using this antibody. Is that expected? Could you tell me where is SRC supposed to be expressed?
Verified Customer
Asked: 2019-02-21
Answer
Based on literature, liver does express SRC. Generally SRC expresses in cell membrane. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-02-21
Question
Is a blocking peptide available for product anti-Phospho-Src (Y419) Monoclonal antibody (MP00107)?
Verified Customer
Asked: 2019-02-06
Answer
We do provide the blocking peptide for product anti-Phospho-Src (Y419) Monoclonal antibody (MP00107). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-06
Question
I have a question about product MP00107, anti-Phospho-Src (Y419) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Asked: 2018-12-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MP00107 anti-Phospho-Src (Y419) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-17
Question
We have tried in the past anti-Phospho-Src (Y419) Monoclonal antibody for Flow Cytometry on leukemic t-cell in the past. I am using human, and We intend to use the antibody for WB next. My lab would like examining leukemic t-cell as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
A. Roberts
Asked: 2018-09-04
Answer
I viewed the website and datasheets of our anti-Phospho-Src (Y419) Monoclonal antibody and it seems that MP00107 has been validated on human in both Flow Cytometry and WB. Thus MP00107 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-04
Question
Is this MP00107 anti-Phospho-Src (Y419) Monoclonal antibody reactive to the isotypes of SRC?
E. Bhatt
Asked: 2018-04-12
Answer
The immunogen of MP00107 anti-Phospho-Src (Y419) Monoclonal antibody is A synthesized peptide derived from human Phospho-Src (Y419). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-12
Question
Do you have a BSA free version of anti-Phospho-Src (Y419) Monoclonal antibody MP00107 available?
Verified Customer
Asked: 2018-03-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Src (Y419) Monoclonal antibody MP00107 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Phospho-Src (Y419) Monoclonal antibody MP00107. Let me know if you need anything else.
Verified Customer
Asked: 2017-12-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-25
Question
My question regards to test anti-Phospho-Src (Y419) Monoclonal antibody MP00107 on human liver for research purposes, then I may be interested in using anti-Phospho-Src (Y419) Monoclonal antibody MP00107 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Miller
Asked: 2017-10-27
Answer
The products we sell, including anti-Phospho-Src (Y419) Monoclonal antibody MP00107, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-27
Question
Here is the WB image, lot number and protocol we used for liver using anti-Phospho-Src (Y419) Monoclonal antibody MP00107. Please let me know if you require anything else.
R. Mitchell
Asked: 2017-05-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-18
Question
I was wanting to use using your anti-Phospho-Src (Y419) Monoclonal antibody for intracellular signal transduction studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
A. Johnson
Asked: 2016-03-30
Answer
Thank you for your inquiry. This MP00107 anti-Phospho-Src (Y419) Monoclonal antibody is validated on a431 cell lysate. It is guaranteed to work for Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-03-30
Question
We have seen staining in human erythroleukemia. Any tips? Is anti-Phospho-Src (Y419) Monoclonal antibody supposed to stain erythroleukemia positively?
V. Krishna
Asked: 2015-10-26
Answer
Based on literature erythroleukemia does express SRC. Based on Uniprot.org, SRC is expressed in fundus of stomach, lung skin, platelet, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, among other tissues. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2015-10-26
Question
I see that the anti-Phospho-Src (Y419) Monoclonal antibody MP00107 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
W. Collins
Asked: 2014-08-22
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-08-22
Question
Does MP00107 anti-Phospho-Src (Y419) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Dhar
Asked: 2014-03-26
Answer
As indicated on the product datasheet, MP00107 anti-Phospho-Src (Y419) Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-03-26
Question
Will anti-Phospho-Src (Y419) Monoclonal antibody MP00107 work for Flow Cytometry with liver?
D. Huang
Asked: 2013-04-05
Answer
According to the expression profile of liver, SRC is highly expressed in liver. So, it is likely that anti-Phospho-Src (Y419) Monoclonal antibody MP00107 will work for Flow Cytometry with liver.
Boster Scientific Support
Answered: 2013-04-05
