Overview
| Product Name |
Anti-Phospho-Src (Y419) Monoclonal Antibody
See all SRC primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
MP00107 |
| Pack Size |
100ug |
| Storage & Handling |
At -20°C for one year. Avoid repeated freezing and thawing. |
| Description |
Rabbit IgG Monoclonal antibody for Phospho-Src (Y419) detection. Tested positive for WB, FC in Human, Mouse, Rat |
| Cite This Product |
Anti-Phospho-Src (Y419) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00107)
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| Antibodies Validation |
Antibodies Validation Information
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Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse, Rat |
| Applications |
Flow Cytometry, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A synthesized peptide derived from human Phospho-Src (Y419) |
Gene/Protein Basic Information For SRC (Source: Uniprot.org, NCBI)
| Uniprot Id | P12931 |
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| NCBI Gene Id | 6714 |
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| Species Of This Entry | Human |
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| Gene Name | SRC |
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| Protein Name | Proto-oncogene tyrosine-protein kinase Src |
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| Superfamily | protein kinase superfamily |
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| Alternative Names | SRC|ASV; c-Src; EC 2.7.10; EC 2.7.10.2; pp60c-src; Rous sarcoma; RSVgp4; Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
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| Molecular Weight | 59835 |
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| Post Tranlational Modifications | Phosphorylation; Dephosphorylation; Cleavage; Myristoylation; Ubiquitination; Methylation; Palmitoylation; Reduction; Acetylation; Oxidation; Biotinylation; Acylation; Glycosylation; Ribosylation; Nitration; Prenylation; Nitrosylation; Farnesylation; Demethylation; Amidation |
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| Subcellular Localization | Cell membrane; Lipid-anchor. Mitochondrion inner membrane. Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell junction, focal adhesion. Localizes to focal adhesion sites following integrin engagement (PubMed:22801373). Localization to focal adhesion sites requires myristoylation and the SH3 domain (PubMed:7525268). Colocalizes with PDLIM4 at the perinuclear region, but not at focal adhesions (PubMed:19307596). |
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| Gene Location | 20q11.23, on Chromasome 20, gene sequence: NC_000020.11 (37344685..37406050) |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For SRC (Source: Uniprot.org, NCBI)
| Protein Function | Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein- coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an important role in the regulation of cytoskeletal organization through phosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 allows the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled through the phosphorylation of cortactin (CTTN) (Probable). When cells adhere via focal adhesions to the extracellular matrix, signals are transmitted by integrins into the cell resulting in tyrosine phosphorylation of a number of focal adhesion proteins, including PTK2/FAK1 and paxillin (PXN) (PubMed:21411625). In addition to phosphorylating focal adhesion proteins, SRC is also active at the sites of cell-cell contact adherens junctions and phosphorylates substrates such as beta- catenin (CTNNB1), delta-catenin (CTNND1), and plakoglobin (JUP). Another type of cell-cell junction, the gap junction, is also a target for SRC, which phosphorylates connexin-43 (GJA1). SRC is implicated in regulation of pre-mRNA-processing and phosphorylates RNA-binding proteins such as KHDRBS1 (Probable). Also plays a role in PDGF-mediated tyrosine phosphorylation of both STAT1 and STAT3, leading to increased DNA binding activity of these transcription factors (By similarity). Involved in the RAS pathway through phosphorylation of RASA1 and RASGRF1 (PubMed:11389730). Plays a role in EGF-mediated calcium-activated chloride channel activation (PubMed:18586953). Required for epidermal growth factor receptor (EGFR) internalization through phosphorylation of clathrin heavy chain (CLTC and CLTCL1) at 'Tyr-1477'. Involved in beta-arrestin (ARRB1 and ARRB2) desensitization through phosphorylation and activation of GRK2, leading to beta-arrestin phosphorylation and internalization. Has a critical role in the stimulation of the CDK20/MAPK3 mitogen-activated protein kinase cascade by epidermal growth factor (Probable). Might be involved not only in mediating the transduction of mitogenic signals at the level of the plasma membrane but also in controlling progression through the cell cycle via interaction with regulatory proteins in the nucleus (PubMed:7853507). Plays an important role in osteoclastic bone resorption in conjunction with PTK2B/PYK2. Both the formation of a SRC-PTK2B/PYK2 complex and SRC kinase activity are necessary for this function. Recruited to activated integrins by PTK2B/PYK2, thereby phosphorylating CBL, which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function (PubMed:8755529, PubMed:14585963). Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase (PubMed:12615910). Phosphorylates DDR2 on tyrosine residues, thereby promoting its subsequent autophosphorylation (PubMed:16186108). Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731' (PubMed:20100835, PubMed:21309750). Enhances DDX58/RIG-I-elicited antiviral signaling (PubMed:19419966). Phosphorylates PDPK1 at 'Tyr-9', 'Tyr-373' and 'Tyr-376' (PubMed:14585963). Phosphorylates BCAR1 at 'Tyr-128' (PubMed:22710723). Phosphorylates CBLC at multiple tyrosine residues, phosphorylation at 'Tyr-341' activates CBLC E3 activity (PubMed:20525694). Involved in anchorage-independent cell growth (PubMed:19307596). Required for podosome formation (By similarity). |
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| Tissue Specificity | Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. |
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| Related Diseases | Malignant Neoplasms; Neoplasms; Tissue Adhesions; Cell Transformation, Neoplastic; Sarcoma; Neoplasm Metastasis; Carcinoma; Cell Invasion; Leukemia; Malignant Neoplasm Of Breast; Mammary Neoplasms; Tumor Angiogenesis; Malignant Paraganglionic Neoplasm; Inflammation; Neoplasm Invasiveness; Metaplastic Cell Transformation; Myeloid Leukemia; Adenocarcinoma |
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| Related Pathways | Cell Proliferation; Localization; Cell Migration; Cell Growth; Cell Adhesion; Cell Cycle; Angiogenesis; Secretion; Endocytosis; Pathogenesis; Cell Activation; Cell Death; Cell Motility; Transport; T Cell Activation; Rna Interference; Translation; Immune Response; Chemotaxis; Cell Differentiation |
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| Scientific References | PMID: 3299057 by Tanaka A., et al. DNA sequence encoding the amino-terminal region of the human c-src protein: implications of sequence divergence among src-type kinase oncogenes.
PMID: 2582238 by Anderson S.K., et al. Human cellular src gene: nucleotide sequence and derived amino acid sequence of the region coding for the carboxy-terminal two-thirds of pp60c-src. |
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Our Boster Quality Guarantee for Anti-Phospho-Src (Y419) Monoclonal Antibody covers its use in the following applications.
WB 1:500~1:2000
FC 1:30
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Western blot analysis of SRC using anti-SRC antibody (MP00107) in A431 cell lysate treated with pervanadate.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRC antigen affinity purified polyclonal antibody (Catalog # MP00107) at 0.5 ug/mL overnight at 4
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 1
| Zhang Qq, Zhou Dl, Lei Y, Zheng L, Chen Sx, Gou Hj, Gu Ql, He Xd, Lan T, Qi Cl, Li Jc, Ding Yq, Qiao L, Wang Lj. Oncotarget. 2015 Feb 20;6(5):3123-35. Slit2/Robo1 Signaling Promotes Intestinal Tumorigenesis Through Src-Mediated Activation Of The W... |
PubMed ID 25605242 |
