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Product Info Summary
| SKU: | P00036 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-STAT1 (Y701) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00036
BM4753 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-STAT1 (Y701) Rabbit Monoclonal Antibody catalog # P00036. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-STAT1 (Y701) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00036)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HHB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human STAT1
Reactive Species
P00036 is reactive to STAT1 in Human
Observed Molecular Weight
87 kDa
Calculated molecular weight
87.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00036 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-STAT1 (Y701) expression in (1) A431 cell lysate; (2) A431 lysate cell treated with EGF.
Specific Publications For Anti-Phospho-STAT1 (Y701) Rabbit Monoclonal Antibody (P00036)
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-STAT1 (Y701) Rabbit Monoclonal Antibody
Question
We need using your anti-Phospho-STAT1 (Y701) Rabbit Monoclonal antibody for interleukin-9-mediated signaling pathway studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
We appreciate your inquiry. This P00036 anti-Phospho-STAT1 (Y701) Rabbit Monoclonal antibody is tested on a431 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-12
Question
We are currently using anti-Phospho-STAT1 (Y701) Rabbit Monoclonal antibody P00036 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
A. Banerjee
Verified customer
Asked: 2019-05-01
Answer
The anti-Phospho-STAT1 (Y701) Rabbit Monoclonal antibody (P00036) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-01
Question
We have seen staining in human cervix carcinoma. Do you have any suggestions? Is anti-Phospho-STAT1 (Y701) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
Based on literature cervix carcinoma does express STAT1. Based on Uniprot.org, STAT1 is expressed in epithelium of bronchus, placenta testis, brain, b-cell, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-12-29
Question
Our lab were happy with the WB result of your anti-Phospho-STAT1 (Y701) Rabbit Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell cytoplasm using this antibody. Is that expected? Could you tell me where is STAT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-06-13
Answer
Based on literature, leukemic t-cell does express STAT1. Generally STAT1 expresses in cytoplasm. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-06-13
