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Product Info Summary
| SKU: | P30917 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho Tensin-2 (Tyr483) Antibody
SKU/Catalog Number
P30917
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho Tensin-2 (Tyr483) Antibody (Catalog# P30917). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho Tensin-2 (Tyr483) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30917)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A phospho-peptide derived from human Tensin-2 around the phosphorylation site of Tyr483.
Reactive Species
P30917 is reactive to TNS2 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
152.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30917 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of 3T3 cells using Phospho-Tensin-2 (Y483) Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of A549 NIH-3T3 SH-SY5Y K562 HELA cells using Phospho-Tensin-2 (Y483) Polyclonal Antibody diluted at 1:2000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tensin-2 (Phospho-Tyr483) Antibody
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Western blot analysis of lysate from K562 cells, using phospho-Thrensin-2 (Phospho-Tyr483) antibody .
Specific Publications For Anti-Phospho Tensin-2 (Tyr483) Antibody (P30917)
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1 Customer Q&As for Anti-Phospho Tensin-2 (Tyr483) Antibody
Question
We are currently using anti-Phospho Tensin-2 (Tyr483) antibody P30917 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
P. Moore
Verified customer
Asked: 2018-06-07
Answer
The anti-Phospho Tensin-2 (Tyr483) antibody (P30917) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-07
