Product Info Summary
SKU: | P01720-2 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-Troponin I, cardiac (Ser43) Antibody
SKU/Catalog Number
P01720-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Troponin I, cardiac (Ser43) Antibody (Catalog # P01720-2). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Troponin I, cardiac (Ser43) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01720-2)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Clone Number
608
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Mouse.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01720-2 is reactive to TNNI3 in Human, Mouse, Rat
Applications
P01720-2 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
24.008kDa
Background of TNNI3
Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser-43 and Ser-45 of cardiac troponin I plays a substantial role in the PKC mediated depression. (Montgomery et al, 2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser43 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 minutes).
Protein Target Info & Infographic
Gene/Protein Information For TNNI3 (Source: Uniprot.org, NCBI)
Gene Name
TNNI3
Full Name
Troponin I, cardiac muscle
Weight
24.008kDa
Superfamily
troponin I family
Alternative Names
Cardiac troponin I; CMD1FF; CMD2A; CMH7MGC116817; cTnI; familial hypertrophic cardiomyopathy 7; RCM1; TNNC1troponin I, cardiac; troponin I type 3 (cardiac); troponin I, cardiac muscle TNNI3 CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI troponin I3, cardiac type troponin I, cardiac muscle|cardiomyopathy, dilated 2A (autosomal recessive)|troponin I type 3 (cardiac)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNNI3, check out the TNNI3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNNI3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Troponin I, cardiac (Ser43) Antibody (P01720-2)
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No publications found for P01720-2
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-Troponin I, cardiac (Ser43) Antibody
Question
We are currently using anti-Phospho-Troponin I, cardiac (Ser43) antibody P01720-2 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The anti-Phospho-Troponin I, cardiac (Ser43) antibody (P01720-2) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-07