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| SKU | MA1042 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Clone Number | GAP-7B10 |
| Host | Mouse |
| Ig Isotype | Mouse IgG2a |
| Applications | IHC, WB |
Overview
| Product Name | Anti-GAP43 Antibody (Monoclonal, GAP-7B10) |
|---|---|
| SKU/Catalog Number | MA1042 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for GAP43, growth associated protein 43 (GAP43) detection. Tested with WB, IHC-P, IHC-F in Human; mouse; rat. No cross reactivity with otherproteins. |
| Cite This Product | Anti-GAP43 Antibody (Monoclonal, GAP-7B10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1042) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Immunogen | GAP-43 from neonatal rat forebrain membranes. |
| Reactivity | Human, Mouse, Rat |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).Images And Assay Conditions
Anti- GAP43 antibody, MA1042, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P07936 |
|---|---|
| Gene Name | GAP43 |
| Protein Name | Neuromodulin |
| Alternative Names | Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Protein F1;Gap43; |
| Subcellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes. |
| Molecular Weight | 23603 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity). . |
|---|---|
| Research Areas | Axonal Guidance Proteins, Cell Type Marker, Growth And Development, Neurology Process, Neuron Marker, Neuroscience, Synapse Marker *You can search these to find other products in these research areas. |
| Background | GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system. |
Other Recommended Resources
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Publications
Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells
Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats
Fructus Corni extract-induced neuritogenesis in PC12 cells is associated with the suppression of stromal interaction molecule 1 expression and inhibition of Ca2+ influx
Hui Liu, Peizhen Lv, Yongjia Zhu, Huayu Wu, Kun Zhang, Fuben Xu, Li Zheng & Jinmin Zhao
Scientific Reports 7, Article number: 39869 (2017)
doi:10.1038/srep39869
Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo
Zou H, Ding Y, Shi W, Xu X, Gong A, Zhang Z, Liu J. Cell Mol Neurobiol. 2015 Apr;35(3):313-22. Doi: 10.1007/S10571-014-0126-X. Epub 2014 Oct 29. Microrna-29C/Pten Pathway Is Involved In Mice Brain Development And Modulates Neurite Outgrowth In Pc1...
Zou H, Ding Y, Wang K, Xiong E, Peng W, Du F, Zhang Z, Liu J, Gong A. Neuroscience. 2015 Apr 16;291:289-300. Doi: 10.1016/J.Neuroscience.2015.01.055. Epub 2015 Feb 7. Microrna-29A/Pten Pathway Modulates Neurite Outgrowth In Pc12 Cells.
Nie X, Deng M, Yang M, Liu L, Zhang Y, Wen X. Cell Biochem Biophys. 2014 Jan;68(1):163-72. Doi: 10.1007/S12013-013-9683-8. Axonal Regeneration And Remyelination Evaluation Of Chitosan/Gelatin-Based Nerve Guide Combined With Transforming Growth Fac...
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu Dh, Deng W, Lai Y, Zhou Jh, Zhang Sx, Lin Ww, Chen Df. Brain Res. 2014 Mar 13;1552:41-54. Doi: 10.1016/J.Brainres.2014.01.005. Epub 2014 Jan 20. ??-Asarone Prevents Autop...
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to gap 43 antibody, gap43 antibody, growth associated protein 43 antibody, growth-associated protein 43 antibody