Anti-GAP43 Antibody (Monoclonal, GAP-7B10)

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SKU MA1042
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number GAP-7B10
Host Mouse
Ig Isotype Mouse IgG2a
Applications IHC, WB

Overview

Product Name Anti-GAP43 Antibody (Monoclonal, GAP-7B10)
SKU/Catalog Number MA1042
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for GAP43, growth associated protein 43 (GAP43) detection. Tested with WB, IHC-P, IHC-F in Human; mouse; rat. No cross reactivity with otherproteins.
Cite This Product Anti-GAP43 Antibody (Monoclonal, GAP-7B10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1042)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen GAP-43 from neonatal rat forebrain membranes.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1042 1 IHC anti gap43 antibody monoclonal gap 7b10.jpg

Anti- GAP43 antibody, MA1042, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P07936
Gene Name GAP43
Protein Name Neuromodulin
Alternative Names Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Protein F1;Gap43;
Subcellular Localization Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes.
Molecular Weight 23603 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity). .
Research Areas Axonal Guidance Proteins, Cell Type Marker, Growth And Development, Neurology Process, Neuron Marker, Neuroscience, Synapse Marker

*You can search these to find other products in these research areas.
Background GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.

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Order Product (MA1042)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships in 7-10 business days.

Troubleshooting

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Publications

Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells
PubMed ID29138751
Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats
PubMed ID26238991
Fructus Corni extract-induced neuritogenesis in PC12 cells is associated with the suppression of stromal interaction molecule 1 expression and inhibition of Ca2+ influx
PubMed ID26136892
Hui Liu, Peizhen Lv, Yongjia Zhu, Huayu Wu, Kun Zhang, Fuben Xu, Li Zheng & Jinmin Zhao Scientific Reports 7, Article number: 39869 (2017) doi:10.1038/srep39869 Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo
PubMed ID-
Zou H, Ding Y, Shi W, Xu X, Gong A, Zhang Z, Liu J. Cell Mol Neurobiol. 2015 Apr;35(3):313-22. Doi: 10.1007/S10571-014-0126-X. Epub 2014 Oct 29. Microrna-29C/Pten Pathway Is Involved In Mice Brain Development And Modulates Neurite Outgrowth In Pc1...
PubMed ID25352418
Zou H, Ding Y, Wang K, Xiong E, Peng W, Du F, Zhang Z, Liu J, Gong A. Neuroscience. 2015 Apr 16;291:289-300. Doi: 10.1016/J.Neuroscience.2015.01.055. Epub 2015 Feb 7. Microrna-29A/Pten Pathway Modulates Neurite Outgrowth In Pc12 Cells.
PubMed ID25665754
Nie X, Deng M, Yang M, Liu L, Zhang Y, Wen X. Cell Biochem Biophys. 2014 Jan;68(1):163-72. Doi: 10.1007/S12013-013-9683-8. Axonal Regeneration And Remyelination Evaluation Of Chitosan/Gelatin-Based Nerve Guide Combined With Transforming Growth Fac...
PubMed ID23740553
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu Dh, Deng W, Lai Y, Zhou Jh, Zhang Sx, Lin Ww, Chen Df. Brain Res. 2014 Mar 13;1552:41-54. Doi: 10.1016/J.Brainres.2014.01.005. Epub 2014 Jan 20. ??-Asarone Prevents Autop...
PubMed ID24457043

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to gap 43 antibody, gap43 antibody, growth associated protein 43 antibody, growth-associated protein 43 antibody