Click image to see more details
Product Info Summary
| SKU: | MA1042 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GAP43 Antibody (Monoclonal, GAP-7B10)
SKU/Catalog Number
MA1042
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GAP43 Antibody (Monoclonal, GAP-7B10) catalog # MA1042. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAP43 Antibody (Monoclonal, GAP-7B10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1042)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
GAP-7B10
Isotype
Mouse IgG2a
Immunogen
GAP-43 from neonatal rat forebrain membranes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins
Reactive Species
MA1042 is reactive to Gap43 in Human, Mouse, Rat
Applications
MA1042 is guaranteed for IHC, WB Boster Guarantee
Background of GAP-43
GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Validation Images & Assay Conditions
Click image to see more details
Anti-GAP43 antibody, MA1042, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Protein Target Info & Infographic
Gene/Protein Information For Gap43 (Source: Uniprot.org, NCBI)
Uniprot ID
P07936
Gene ID
29423
Gene Name
Gap43
Full Name
Neuromodulin
Weight
23.603kDa
Superfamily
neuromodulin family
Alternative Names
Axonal membrane protein GAP-43; B-50; Basp2; calmodulin-binding protein P-57; GAP43; GAP-43; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; PP46; protein F1 Gap43|Basp2|growth associated protein 43|neuromodulin|axonal membrane protein GAP-43|brain abundant, membrane attached signal protein 2|growth accentuating protein 43|protein F1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Gap43, check out the Gap43 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Gap43: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
Specific Publications For Anti-GAP43 Antibody (Monoclonal, GAP-7B10) (MA1042)
MA1042 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lin J,Hao C,Gong Y,Zhang Y,Li Y,Feng Z,Xu X,Huang H,Liao W. Effect of Tetramethylpyrazine on Neuroplasticity after Transient Focal Cerebral Ischemia Reperfusion in Rats. Evid Based Complement Alternat Med. 2021 Jan 18;2021:1587241.doi:10.1155/2021/1587241
Species: Rat
MA1042 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Shi W, Bian L, Lv D, Bi S, Dai Y, Yang K, Lu H, Zhou H, Que Y, Wang D, Zhang Z, Lu N. Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro. Amino Acids. 2020 Nov 27. doi: 10.1007/
Species: Rat,Adenovirus
MA1042 usage in article: APP:IF, SAMPLE:NSCS, DILUTION:1:250
Bone marrow stromal cells improved functional recovery in spinal cord injury rats partly via the Toll‑like receptor‑4/nuclear factor‑κB signaling pathway Shi Bai, Hao Zhou, and Lijuan Wu Exp Ther Med. 2019 Jan; 17(1): 444–448. Published online 2018 Oct
Fructus Corni extract-induced neuritogenesis in PC12 cells is associated with the suppression of stromal interaction molecule 1 expression and inhibition of Ca2+ influx
Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats
Liu Z, Liu H, Zeng ZH. Biomed Res Int. 2018 Mar 7;2018:8697913. doi: 10.1155/2018/8697913. eCollection 2018. Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Incre...
Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo
Zou H, Ding Y, Shi W, Xu X, Gong A, Zhang Z, Liu J. Cell Mol Neurobiol. 2015 Apr;35(3):313-22. Doi: 10.1007/S10571-014-0126-X. Epub 2014 Oct 29. Microrna-29C/Pten Pathway Is Involved In Mice Brain Development And Modulates Neurite Outgrowth In Pc1...
Zou H, Ding Y, Wang K, Xiong E, Peng W, Du F, Zhang Z, Liu J, Gong A. Neuroscience. 2015 Apr 16;291:289-300. Doi: 10.1016/J.Neuroscience.2015.01.055. Epub 2015 Feb 7. Microrna-29A/Pten Pathway Modulates Neurite Outgrowth In Pc12 Cells.
Nie X, Deng M, Yang M, Liu L, Zhang Y, Wen X. Cell Biochem Biophys. 2014 Jan;68(1):163-72. Doi: 10.1007/S12013-013-9683-8. Axonal Regeneration And Remyelination Evaluation Of Chitosan/Gelatin-Based Nerve Guide Combined With Transforming Growth Fac...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-GAP43 Antibody (Monoclonal, GAP-7B10)
Question
Is MA1042 able to cross-react with rabbit?
Verified customer
Asked: 2021-11-09
Answer
Unfortunately, we are uncertain if the Anti-GAP43 Antibody (Monoclonal, GAP-7B10) (MA1042) is able to cross-react with rabbits. We suggest running a pilot test to verify this.
Boster Scientific Support
Answered: 2021-11-10
Question
I was wanting to use your anti-GAP43 antibody (Monoclonal, GAP-7B10) for WB for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
You can see on the product datasheet, MA1042 anti-GAP43 antibody (Monoclonal, GAP-7B10) has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-31
Question
My lab would like using your anti-GAP43 antibody (Monoclonal, GAP-7B10) for axon choice point recognition studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We appreciate your inquiry. This MA1042 anti-GAP43 antibody (Monoclonal, GAP-7B10) is validated on cardiac muscle tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-20
Question
We have observed staining in human brain. What should we do? Is anti-GAP43 antibody (Monoclonal, GAP-7B10) supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
According to literature brain does express GAP43. According to Uniprot.org, GAP43 is expressed in anterior cingulate cortex, brain cortex subthalamic nucleus, brain, among other tissues. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-GAP43 antibody (Monoclonal, GAP-7B10) (MA1042)?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
We do provide the blocking peptide for product anti-GAP43 antibody (Monoclonal, GAP-7B10) (MA1042). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-23
Question
My question regarding product MA1042, anti-GAP43 antibody (Monoclonal, GAP-7B10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1042 anti-GAP43 antibody (Monoclonal, GAP-7B10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-05
Question
I see that the anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]m
Boster Scientific Support
Answered: 2019-11-18
Question
Is this MA1042 anti-GAP43 antibody (Monoclonal, GAP-7B10) reactive to the isotypes of GAP43?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The immunogen of MA1042 anti-GAP43 antibody (Monoclonal, GAP-7B10) is GAP-43 from neonatal rat forebrain membranes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-05
Question
We are currently using anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The anti-GAP43 antibody (Monoclonal, GAP-7B10) (MA1042) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-05
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-20
Question
We are interested in to test anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042 on mouse brain for research purposes, then I may be interested in using anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The products we sell, including anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-20
Question
Will MA1042 anti-GAP43 antibody (Monoclonal, GAP-7B10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Carter
Verified customer
Asked: 2019-02-26
Answer
You can see on the product datasheet, MA1042 anti-GAP43 antibody (Monoclonal, GAP-7B10) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-26
Question
Please see the WB image, lot number and protocol we used for brain using anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-26
Question
Our team were well pleased with the WB result of your anti-GAP43 antibody (Monoclonal, GAP-7B10). However we have been able to see positive staining in anterior cingulate cortex cell membrane using this antibody. Is that expected? Could you tell me where is GAP43 supposed to be expressed?
K. Singh
Verified customer
Asked: 2017-11-29
Answer
From what I have seen in literature, anterior cingulate cortex does express GAP43. Generally GAP43 expresses in cell membrane. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-11-29
Question
Is there a BSA free version of anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042 available?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-23
Question
We ordered your anti-GAP43 antibody (Monoclonal, GAP-7B10) for IHC on anterior cingulate cortex in a previous experiment. I am using rat, and We are going to use the antibody for WB next. I am looking for examining anterior cingulate cortex as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for WB?
R. Singh
Verified customer
Asked: 2017-04-11
Answer
I looked at the website and datasheets of our anti-GAP43 antibody (Monoclonal, GAP-7B10) and it seems that MA1042 has been validated on rat in both IHC and WB. Thus MA1042 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-04-11
Question
Does anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042 work for WB with brain?
G. Wu
Verified customer
Asked: 2013-08-01
Answer
According to the expression profile of brain, GAP43 is highly expressed in brain. So, it is likely that anti-GAP43 antibody (Monoclonal, GAP-7B10) MA1042 will work for WB with brain.
Boster Scientific Support
Answered: 2013-08-01
