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Anti-GAP43 Antibody (Monoclonal, GAP-7B10)
Mouse IgG monoclonal antibody for GAP43, growth associated protein 43 (GAP43) detection. Tested with WB, IHC-P, IHC-F in Human; mouse; rat. No cross reactivity with otherproteins.
| SKU | MA1042 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Clone Number | GAP-7B10 |
| Host | Mouse |
| Ig Isotype | Mouse IgG2a |
| Applications | IHC, WB |
Overview
| Product Name | Anti-GAP43 Antibody (Monoclonal, GAP-7B10) See all Gap43 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | MA1042 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Monoclonal clone# G2 antibody for GAP-43/GAP43 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. GAP-43/GAP43 information: Molecular Weight: 23603 MW; Subcellular Localization: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes. |
| Cite This Product | Anti-GAP43 Antibody (Monoclonal, GAP-7B10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1042) |
| Specificity | Anti-GAP43 Antibody (Monoclonal, GAP-7B10) (MA1042) reacts with Human, Mouse, Rat Gap43, in native form and recombinant. Superfamily members of Gap43 are not reactive to MA1042. |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Form | Lyophilized |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Immunogen | GAP-43 from neonatal rat forebrain membranes. |
| Reactivity | Human, Mouse, Rat |
Assay Details
Our Boster Quality Guarantee for Anti-GAP43 Antibody (Monoclonal, GAP-7B10) covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-GAP43 Antibody (Monoclonal, GAP-7B10) (MA1042).
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).Images And Assay Conditions
Anti- GAP43 antibody, MA1042, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P07936 |
|---|---|
| Gene Name | Gap43 |
| Protein Name | Neuromodulin |
| Alternative Names | Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Protein F1;Gap43; |
| Subcellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes. |
| Molecular Weight | 23603 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity). . |
|---|---|
| Research Areas | Human, Mouse, Rat *You can search these to find other products in these research areas. |
| Background | GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system. |
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Publishing with Anti-GAP43 Antibody (Monoclonal, GAP-7B10) (MA1042)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]
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MA1042
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
Publications
Fructus Corni extract-induced neuritogenesis in PC12 cells is associated with the suppression of stromal interaction molecule 1 expression and inhibition of Ca2+ influx
Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats
Liu Z, Liu H, Zeng ZH. Biomed Res Int. 2018 Mar 7;2018:8697913. doi: 10.1155/2018/8697913. eCollection 2018. Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Incre...
Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo
Zou H, Ding Y, Shi W, Xu X, Gong A, Zhang Z, Liu J. Cell Mol Neurobiol. 2015 Apr;35(3):313-22. Doi: 10.1007/S10571-014-0126-X. Epub 2014 Oct 29. Microrna-29C/Pten Pathway Is Involved In Mice Brain Development And Modulates Neurite Outgrowth In Pc1...
Zou H, Ding Y, Wang K, Xiong E, Peng W, Du F, Zhang Z, Liu J, Gong A. Neuroscience. 2015 Apr 16;291:289-300. Doi: 10.1016/J.Neuroscience.2015.01.055. Epub 2015 Feb 7. Microrna-29A/Pten Pathway Modulates Neurite Outgrowth In Pc12 Cells.
Nie X, Deng M, Yang M, Liu L, Zhang Y, Wen X. Cell Biochem Biophys. 2014 Jan;68(1):163-72. Doi: 10.1007/S12013-013-9683-8. Axonal Regeneration And Remyelination Evaluation Of Chitosan/Gelatin-Based Nerve Guide Combined With Transforming Growth Fac...
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu Dh, Deng W, Lai Y, Zhou Jh, Zhang Sx, Lin Ww, Chen Df. Brain Res. 2014 Mar 13;1552:41-54. Doi: 10.1016/J.Brainres.2014.01.005. Epub 2014 Jan 20. ??-Asarone Prevents Auto...
Customer Q&As
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
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Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
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Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
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Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to gap 43 antibody, gap43 antibody, growth associated protein 43 antibody, growth-associated protein 43 antibody