|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-GAP43 Antibody (Monoclonal, GAP-7B10)|
|Description||Mouse IgG monoclonal antibody for GAP43, growth associated protein 43 (GAP43) detection. Tested with WB, IHC-P, IHC-F in Human; mouse; rat. No cross reactivity with otherproteins.|
|Cite This Product||Anti-GAP43 Antibody (Monoclonal, GAP-7B10) GAP-7B10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1042)|
|Replacement Item||This antibody may replace the following items: sc-10786|sc-135697|sc-135915|sc-17109|sc-17109-R|sc-17790|sc-33705|sc-7457|sc-7458 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||GAP-43 from neonatal rat forebrain membranes.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||23603 MW|
|Protein Function||This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity). .|
|Sequence Similarities||Belongs to the neuromodulin family.|
|Subcellular Localization||Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes.|
|Alternative Names||Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Protein F1;Gap43;|
|Research Areas|||neuroscience|cell type marker|neuron marker|growth cone| neuroscience|synapse marker|neurology process|growth and development|axonal guidance proteins||
Background for Neuromodulin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-GAP43 Antibody (Monoclonal, GAP-7B10) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-GAP43 Antibody (Monoclonal, GAP-7B10) Images
Click the images to enlarge.
IHC(P): Rat Cardiac Muscle Tissue